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PMID: 10831415 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Phylogenetic relationships of Cryptosporidium parasites based on the 70-kilodalton heat shock protein (HSP70) gene.

Applied and environmental microbiology ·Vol. 66 ·No. 6 ·2000-06-00 ·Pages 2385-91

Sulaiman IM, Morgan UM, Thompson RC, Lal AA, Xiao L

Abstract

We have characterized the nucleotide sequences of the 70-kDa heat shock protein (HSP70) genes of Cryptosporidium baileyi, C. felis, C. meleagridis, C. muris, C. serpentis, C. wrairi, and C. parvum from various animals. Results of the phylogenetic analysis revealed the presence of several genetically distinct species in the genus Cryptosporidium and eight distinct genotypes within the species C. parvum. Some of the latter may represent cryptic species. The phylogenetic tree constructed from these sequences is in agreement with our previous results based on the small-subunit rRNA genes of Cryptosporidium parasites. The Cryptosporidium species formed two major clades: isolates of C. muris and C. serpentis formed the first major group, while isolates of C. felis, C. meleagridis, C. wrairi, and eight genotypes of C. parvum formed the second major group. Sequence variations were also observed between C. muris isolates from ruminants and rodents. The HSP70 gene provides another useful locus for phylogenetic analysis of the genus Cryptosporidium.

MeSH Terms
Animals Base Sequence Cryptosporidiosis/parasitology Cryptosporidium/classification,genetics,isolation & purification Feces/parasitology Genes, Protozoan HSP70 Heat-Shock Proteins/genetics Humans Molecular Sequence Data Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA
Chemicals
HSP70 Heat-Shock Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sulaiman I M
Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Services, U.S. Department of Health and Human Services, Atlanta, Georgia 30341, USA.
Morgan U M
Thompson R C
Lal A A
Xiao L
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.66.6.2385-2391.2000
Published
2000-06-00
Pages
2385-91
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC110539
Subset
IM
Databases
GENBANK
AF221528, AF221529, AF221530, AF221531
External Links
PubMed source DOI: 10.1128/AEM.66.6.2385-2391.2000 PMC Full Text View journal details
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