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PMID: 9866750 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Differentiating human from animal isolates of Cryptosporidium parvum.

Emerging infectious diseases ·Vol. 4 ·No. 4 ·1998-00-00 ·Pages 681-5

Sulaiman IM, Xiao L, Yang C, Escalante L, Moore A, Beard CB, Arrowood MJ, Lal AA

Abstract

We analyzed 92 Cryptosporidium parvum isolates from humans and animals by a polymerase chain reaction/restriction fragment length polymorphism method based on the thrombospondin-related anonymous protein 2 gene sequence. Used as a molecular marker, this method can differentiate between the two genotypes of C. parvum and elucidate the transmission of infection to humans.

MeSH Terms

Animals Cattle Cattle Diseases/microbiology Cryptosporidiosis/epidemiology,parasitology,veterinary Cryptosporidium parvum/classification,genetics,isolation & purification Disease Outbreaks Genotype Humans Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Protozoan Proteins/genetics Sequence Analysis, DNA

Chemicals

Protozoan Proteins thrombospondin-related adhesive protein, protozoan

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Sulaiman I M

Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Xiao L

Yang C

Escalante L

Moore A

Beard C B

Arrowood M J

Lal A A

References (4)

4 references, click to expand

Environmental aspects of Cryptosporidium species: a review.
J R Soc Med. 1990 Oct;83(10):629-31 PMID: 2286962
PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin.
FEMS Microbiol Lett. 1997 May 15;150(2):209-17 PMID: 9170264
Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis.
Am J Trop Med Hyg. 1995 Jun;52(6):559-64 PMID: 7611565
Isolation, sequence and molecular karyotype analysis of the actin gene of Cryptosporidium parvum.
Mol Biochem Parasitol. 1992 Jan;50(1):105-13 PMID: 1542305

Article Info

Journal

Emerging infectious diseases

Abbr.

Emerg Infect Dis

ISSN

1080-6040

DOI

10.3201/eid0404.980424

Published

1998-00-00

Pages

681-5

Language

English

Region

United States

NLM ID

9508155

PMCID

PMC2640237

Subset

External Links

PubMed source DOI: 10.3201/eid0404.980424 PMC Full Text View journal details

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Differentiating human from animal isolates of Cryptosporidium parvum.

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