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PMID: 8990285 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequencing of heat shock protein 70 (DnaK) homologs from Deinococcus proteolyticus and Thermomicrobium roseum and their integration in a protein-based phylogeny of prokaryotes.

Journal of bacteriology ·Vol. 179 ·No. 2 ·1997-01-00 ·Pages 345-57

Gupta RS, Bustard K, Falah M, Singh D

Abstract

The 70-kDa heat shock protein (hsp70) sequences define one of the most conserved proteins known to date. The hsp70 genes from Deinococcus proteolyticus and Thermomicrobium roseum, which were chosen as representatives of two of the most deeply branching divisions in the 16S rRNA trees, were cloned and sequenced. hsp70 from both these species as well as Thermus aquaticus contained a large insert in the N-terminal quadrant, which has been observed before as a unique characteristic of gram-negative eubacteria and eukaryotes and is not found in any gram-positive bacteria or archaebacteria. Phylogenetic analysis of hsp70 sequences shows that all of the gram-negative eubacterial species examined to date (which includes members from the genera Deinococcus and Thermus, green nonsulfur bacteria, cyanobacteria, chlamydiae, spirochetes, and alpha-, beta-, and gamma-subdivisions of proteobacteria) form a monophyletic group (excluding eukaryotic homologs which are derived from this group via endosybitic means) strongly supported by the bootstrap scores. A closer affinity of the Deinococcus and Thermus species to the cyanobacteria than to the other available gram-negative sequences is also observed in the present work. In the hsp7O trees, D. proteolyticus and T. aquaticus were found to be the most deeply branching species within the gram-negative eubacteria. The hsp70 homologs from gram-positive bacteria branched separately from gram-negative bacteria and exhibited a closer relationship to and shared sequence signatures with the archaebacteria. A polyphyletic branching of archaebacteria within gram-positive bacteria is strongly favored by different phylogenetic methods. These observations differ from the rRNA-based phylogenies where both gram-negative and gram-positive species are indicated to be polyphyletic. While it remains unclear whether parts of the genome may have variant evolutionary histories, these results call into question the general validity of the currently favored three-domain dogma.

MeSH Terms
Amino Acid Sequence Animals Bacteria/classification,genetics Bacterial Proteins/classification,genetics Base Sequence Cloning, Molecular Codon DNA, Bacterial Gram-Negative Bacteria/classification,genetics Gram-Positive Bacteria/classification,genetics HSP70 Heat-Shock Proteins/classification,genetics Humans Molecular Sequence Data Phylogeny Sequence Homology, Amino Acid
Chemicals
Bacterial Proteins Codon DNA, Bacterial HSP70 Heat-Shock Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gupta R S
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
Bustard K
Falah M
Singh D
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.2.345-357.1997
Published
1997-01-00
Pages
345-57
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC178703
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.179.2.345-357.1997 PMC Full Text View journal details
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