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PMID: 1987088 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequence analysis of the gene encoding the Chlamydia pneumoniae DnaK protein homolog.

Infection and immunity ·Vol. 59 ·No. 2 ·1991-02-00 ·Pages 721-5

Kornak JM, Kuo CC, Campbell LA

Abstract

The antigen-coding region of a 4.2-kb PstI fragment of Chlamydia pneumoniae (pLC3), which encodes a 75-kDa immunoreactive protein recognized during human C. pneumoniae infection, was localized to a 2.0-kb EcoRI fragment. This subclone expressed an immunoreactive fusion protein of ca. 82 kDa. Nucleotide sequence analysis of the C. pneumoniae gene revealed that it consisted of a 1,980-base open reading frame with an inferred 71,550-Da protein of 660 amino acids. Putative Escherichia coli-like promoters and a ribosomal binding site were located in the 5' upstream region, and an 11-base dyad forming a stable stem-loop structure following two in-frame stop codons was identified. The C. pneumoniae 75-kDa protein is a member of the hsp70 family of heat shock proteins and has 87% amino acid similarity with the Chlamydia trachomatis protein.

MeSH Terms

Amino Acid Sequence Bacterial Proteins/analysis,genetics,immunology Base Sequence Chlamydia/genetics Chlamydia trachomatis/genetics Escherichia coli Proteins Genes, Bacterial HSP70 Heat-Shock Proteins Heat-Shock Proteins/analysis,genetics,immunology Molecular Sequence Data Promoter Regions, Genetic

Chemicals

Bacterial Proteins Escherichia coli Proteins HSP70 Heat-Shock Proteins Heat-Shock Proteins dnaK protein, E coli

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kornak J M

Department of Pathobiology, University of Washington, Seattle 98195.

Kuo C C

Campbell L A

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.59.2.721-725.1991

Published

1991-02-00

Pages

721-5

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC257820

Subset

Grants

NIAID NIH HHS · AI 21885 · United States

NEI NIH HHS · EY 00219 · United States

Databases

GENBANK

M69227, S66278, S70201, S70204, X14442, X16435, X53033, X57105, X59240, X60068

External Links

PubMed source DOI: 10.1128/iai.59.2.721-725.1991 PMC Full Text View journal details

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Sequence analysis of the gene encoding the Chlamydia pneumoniae DnaK protein homolog.

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