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PMID: 108262 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Morphological and physiological study of autolytic-defective Streptococcus faecium strains.

Journal of bacteriology ·Vol. 138 ·No. 2 ·1979-05-00 ·Pages 598-608

Shungu DL, Cornett JB, Shockman GD

Abstract

Three autolytic-defective mutants of Streptococcus faecium (S. faecalis ATCC 9790) were isolated. All three autolytic-defective mutants exhibited the following properties relative to the parental strain: (i) slower growth rates, especially in chemically defined medium; (ii) decreased rates of cellular autolysis and increased survival after exposure to antibiotics which block cell wall biosynthesis; (iii) decreased rates of cellular autolysis when treated with detergents, suspended in autolysis buffers, or grown in medium lacking essential cell wall precursors; (iv) a reduction in the total level of cellular autolytic enzyme (active plus latent forms of the enzyme); (v) an increased ratio of latent to active forms of autolysin; and (vi) increased levels of both cellular lipoteichoic acid and lipids.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacteriolysis Cell Wall/drug effects Culture Media Enterococcus faecalis/genetics,physiology,ultrastructure Hydrolases/genetics Lipids/analysis Mutation Surface-Active Agents/pharmacology Teichoic Acids/analysis
Chemicals
Anti-Bacterial Agents Culture Media Lipids Surface-Active Agents Teichoic Acids Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shungu D L
Cornett J B
Shockman G D
References (44)
44 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.138.2.598-608.1979
Published
1979-05-00
Pages
598-608
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218217
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.138.2.598-608.1979 PMC Full Text View journal details
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