Mutant of Bacillus subtilis with a temperature-sensitive autolytic amidase.

Journal of bacteriology ·Vol. 114 ·No. 2 ·1973-05-00 ·Pages 798-803

Fan DP, Beckmann MM

Abstract

A procedure incorporating a petri plate assay for autolysins has been developed for the systematic isolation of mutants with a temperature-sensitive autolytic amidase. We suggest maa as the name for the gene for this enzyme. One such mutant has been characterized as having a heat-sensitive amidase in a variety of studies with both crude autolysin preparations and purified amidase solutions.

MeSH Terms

Alanine Amidohydrolases/isolation & purification,metabolism Autolysis Bacillus subtilis/enzymology,growth & development Cell Wall/metabolism Centrifugation, Density Gradient Chlorides Genes Glucosidases/isolation & purification,metabolism Hot Temperature Lithium Micrococcus Mutagens Mutation Nitrosoguanidines Pronase Sodium Dodecyl Sulfate Temperature

Chemicals

Chlorides Mutagens Nitrosoguanidines Sodium Dodecyl Sulfate Lithium Glucosidases Pronase Amidohydrolases Alanine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Fan D P

Beckmann M M

References (7)

7 references, click to expand

Cell wall binding properties of the Bacillus subtilis autolysin(s).
J Bacteriol. 1970 Aug;103(2):488-93 PMID: 4988245
Autolysin(s) of Bacillus subtilis as dechaining enzyme.
J Bacteriol. 1970 Aug;103(2):494-9 PMID: 4988246
Mutant of Bacillus subtilis demonstrating the requirement of lysis for growth.
J Bacteriol. 1971 Feb;105(2):629-36 PMID: 4993341
Autolytic enzymes in growth of bacteria.
Nature. 1971 Jan 22;229(5282):272-3 PMID: 4927203
Ultrastructural studies on a mutant of Bacillus subtilis whose growth is inhibited due to insufficient autolysin production.
J Bacteriol. 1972 Mar;109(3):1247-57 PMID: 4622130
New centrifugation technique for isolating enzymes from large cell structures: isolation and characterization of two Bacillus subtilis autolysins.
J Bacteriol. 1972 Mar;109(3):1258-65 PMID: 4622131
Micrococcus lysodeikticus bacterial walls as a substrate specific for the autolytic glycosidase of Bacillus subtilis.
J Bacteriol. 1973 May;114(2):804-13 PMID: 4196257

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.114.2.798-803.1973

Published

1973-05-00

Pages

798-803

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC251841

Subset

External Links

PubMed source DOI: 10.1128/jb.114.2.798-803.1973 PMC Full Text View journal details

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Mutant of Bacillus subtilis with a temperature-sensitive autolytic amidase.

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