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PMID: 10734204 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The repertoire of DNA-binding transcriptional regulators in Escherichia coli K-12.

Nucleic acids research ·Vol. 28 ·No. 8 ·2000-04-15 ·Pages 1838-47

Pérez-Rueda E, Collado-Vides J

Abstract

Using a combination of several approaches we estimated and characterized a total of 314 regulatory DNA-binding proteins in Escherichia coli, which might represent its minimal set of transcription factors. The collection is comprised of 35% activators, 43% repressors and 22% dual regulators. Within many regulatory protein families, the members are homogeneous in their regulatory roles, physiology of regulated genes, regulatory function, length and genome position, showing that these families have evolved homogeneously in prokaryotes, particularly in E.coli. This work describes a full characterization of the repertoire of regulatory interactions in a whole living cell. This repertoire should contribute to the interpretation of global gene expression profiles in both prokaryotes and eukaryotes.

MeSH Terms
DNA-Binding Proteins/chemistry,genetics Escherichia coli/genetics Gene Expression Regulation, Bacterial Genes, Bacterial Helix-Turn-Helix Motifs Transcription Factors/chemistry,genetics
Chemicals
DNA-Binding Proteins Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pérez-Rueda E
Programa de Biología Molecular Computacional, Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, AP 565-A 62110, Mexico.
Collado-Vides J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/28.8.1838
Published
2000-04-15
Pages
1838-47
Language
English
Region
England
NLM ID
0411011
PMCID
PMC102813
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/28.8.1838 PMC Full Text View journal details
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