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PMID: 1067607 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Preparative fractionation of DNA restriction fragments by reversed phase column chromatography.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 9 ·1976-09-00 ·Pages 3117-21

Hardies SC, Wells RD

Abstract

Reversed phase column chromatography on RPC-5 resin was used to fractionate milligram quantities of DNA fragments generated by restriction endonucleases. Fractionation was on the basis of size, the presence or absence of sticky ends, and at least one as yet undertermined property.

MeSH Terms
Chromatography Coliphages DNA Restriction Enzymes/metabolism DNA, Bacterial/analysis DNA, Viral/analysis Endonucleases/metabolism Methods
Chemicals
DNA, Bacterial DNA, Viral Endonucleases DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hardies S C
Wells R D
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.9.3117
Published
1976-09-00
Pages
3117-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430949
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.9.3117 PMC Full Text View journal details
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