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PMID: 10675323 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase.

The EMBO journal ·Vol. 19 ·No. 4 ·2000-02-15 ·Pages 542-9

Scotti PA, Urbanus ML, Brunner J, de Gier JW, von Heijne G, van der Does C, Driessen AJ, Oudega B, Luirink J

Abstract

In Escherichia coli, both secretory and inner membrane proteins initially are targeted to the core SecYEG inner membrane translocase. Previous work has also identified the peripherally associated SecA protein as well as the SecD, SecF and YajC inner membrane proteins as components of the translocase. Here, we use a cross-linking approach to show that hydrophilic portions of a co-translationally targeted inner membrane protein (FtsQ) are close to SecA and SecY, suggesting that insertion takes place at the SecA/Y interface. The hydrophobic FtsQ signal anchor sequence contacts both lipids and a novel 60 kDa translocase-associated component that we identify as YidC. YidC is homologous to Saccharomyces cerevisiae Oxa1p, which has been shown to function in a novel export pathway at the mitochondrial inner membrane. We propose that YidC is involved in the insertion of hydrophobic sequences into the lipid bilayer after initial recognition by the SecAYEG translocase.

MeSH Terms
Adenosine Triphosphatases/chemistry,genetics,metabolism Bacterial Proteins/chemistry,genetics,metabolism Binding Sites Carrier Proteins/chemistry,genetics,metabolism Electron Transport Complex IV Escherichia coli/genetics,metabolism Escherichia coli Proteins Fungal Proteins/metabolism Lipid Metabolism Macromolecular Substances Membrane Proteins/chemistry,genetics,metabolism Membrane Transport Proteins Mitochondria/metabolism Mitochondrial Proteins Models, Biological Models, Molecular Mutagenesis, Site-Directed Nuclear Proteins/chemistry,genetics,metabolism SEC Translocation Channels Saccharomyces cerevisiae/metabolism SecA Proteins
Chemicals
Bacterial Proteins Carrier Proteins Escherichia coli Proteins FtsQ protein, E coli Fungal Proteins Macromolecular Substances Membrane Proteins Membrane Transport Proteins Mitochondrial Proteins Nuclear Proteins OXA1 protein SEC Translocation Channels Electron Transport Complex IV Adenosine Triphosphatases SecA Proteins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Scotti P A
Department of Microbiology, Institute of Molecular Biological Sciences, Biocentrum Amsterdam, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands.
Urbanus M L
Brunner J
de Gier J W
von Heijne G
van der Does C
Driessen A J
Oudega B
Luirink J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.4.542
Published
2000-02-15
Pages
542-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305592
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.4.542 PMC Full Text View journal details
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