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PMID: 1066686 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Detection of mRNA sequences in nuclear 30S ribonucleoprotein subcomplexes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 8 ·1976-08-00 ·Pages 2725-9

Kinniburgh AJ, Martin TE

Abstract

RNA from nuclear 30S ribonucleoprotein (RNP) complexes of mouse ascites cells has been shows to contain sequences homologous to poly(A) + mRNA by its ability to hybridize with complementary DNA prepared from poly(A) + mRNA template. Analysis of the hybridization kinetics of poly(A) + mRNA with its own complementary DNA revealed several abundancy classes. The total complexity of poly(A) + mRNA from ascites cells was estimated to be approximately 30,000 sequences of average molecular weight (6 X 10(5)). When the hybridization reaction of 30S RNP-RNA with mRNA-specific cDNA was compared to the homologous reaction the majority, and most probably all, of the poly(A) + mRNA sequences were found to be present in the RNA. The kinetics of hybridization suggest that 10-15% of the RNA in this RNP complex is homologous to poly(A) + mRNA. The 30S RNP subcomplexes therefore contain nuclear poly(A) + mRNA sequences as well as the bulk of heterogeneous RNA.

MeSH Terms
Base Sequence Cell Nucleus Cells, Cultured Nucleoproteins/analysis Poly A/analysis RNA, Messenger/analysis Ribonucleoproteins/analysis
Chemicals
Nucleoproteins RNA, Messenger Ribonucleoproteins Poly A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kinniburgh A J
Martin T E
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.8.2725
Published
1976-08-00
Pages
2725-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430721
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.8.2725 PMC Full Text View journal details
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