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PMID: 10662770 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation of a ribonucleoprotein complex involved in mRNA localization in Drosophila oocytes.

The Journal of cell biology ·Vol. 148 ·No. 3 ·2000-02-07 ·Pages 427-40

Wilhelm JE, Mansfield J, Hom-Booher N, Wang S, Turck CW, Hazelrigg T, Vale RD

Abstract

Localization of bicoid (bcd) mRNA to the anterior and oskar (osk) mRNA to the posterior of the Drosophila oocyte is critical for embryonic patterning. Previous genetic studies implicated exuperantia (exu) in bcd mRNA localization, but its role in this process is not understood. We have biochemically isolated Exu and show that it is part of a large RNase-sensitive complex that contains at least seven other proteins. One of these proteins was identified as the cold shock domain RNA-binding protein Ypsilon Schachtel (Yps), which we show binds directly to Exu and colocalizes with Exu in both the oocyte and nurse cells of the Drosophila egg chamber. Surprisingly, the Exu-Yps complex contains osk mRNA. This biochemical result led us to reexamine the role of Exu in the localization of osk mRNA. We discovered that exu-null mutants are defective in osk mRNA localization in both nurse cells and the oocyte. Furthermore, both Exu/Yps particles and osk mRNA follow a similar temporal pattern of localization in which they transiently accumulate at the oocyte anterior and subsequently localize to the posterior pole. We propose that Exu is a core component of a large protein complex involved in localizing mRNAs both within nurse cells and the developing oocyte.

MeSH Terms
Amino Acid Sequence Animals Binding Sites DNA-Binding Proteins/genetics,isolation & purification,metabolism Drosophila Proteins Drosophila melanogaster/genetics,metabolism Egg Proteins/genetics,isolation & purification,metabolism Female Humans Insect Proteins/genetics Molecular Sequence Data Mutagenesis Oocytes/metabolism Oogenesis RNA, Messenger/metabolism RNA-Binding Proteins/genetics,isolation & purification,metabolism Ribonuclease, Pancreatic/metabolism Ribonucleoproteins/genetics,isolation & purification,metabolism Sequence Homology, Amino Acid
Chemicals
DNA-Binding Proteins Drosophila Proteins Egg Proteins Insect Proteins RNA, Messenger RNA-Binding Proteins Ribonucleoproteins YPS protein, Drosophila exu protein, Drosophila osk protein, Drosophila Ribonuclease, Pancreatic
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wilhelm J E
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California 94143, USA.
Mansfield J
Hom-Booher N
Wang S
Turck C W
Hazelrigg T
Vale R D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.148.3.427
Published
2000-02-07
Pages
427-40
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2174796
Subset
IM
Grants
NIGMS NIH HHS · R01 GM038499 · United States
NIGMS NIH HHS · GM48060 · United States
NIGMS NIH HHS · GM48499 · United States
External Links
PubMed source DOI: 10.1083/jcb.148.3.427 PMC Full Text View journal details
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