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PMID: 9365254 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification and developmental characterization of a novel Y-box protein from Drosophila melanogaster.

Nucleic acids research ·Vol. 25 ·No. 23 ·1997-12-01 ·Pages 4764-70

Thieringer HA, Singh K, Trivedi H, Inouye M

Abstract

The Y-box proteins are a family of highly conserved nucleic acid binding proteins which are conserved from bacteria to human. In this report we have identified a new member of this family from Drosophila melanogaster. Degenerate-PCR was used to identify a conserved region within the highly conserved cold-shock domain (CSD) of Y-box proteins. Subsequently, the cDNA for this gene was sequenced, and the identified open reading frame was named ypsilon schachtel (yps). The expression pattern of yps indicates that this gene is expressed throughout development with the highest level of expression found in adult flies. In situ hybridization shows that the yps mRNA is maternally loaded into the egg cytoplasm. In addition, there appears to be expression of yps mRNA in mesodermal tissue during embryogenesis. YPS, while containing a conserved CSD, is novel in that it completely lacks the alternating acidic and basic regions found in the C-terminus of the other vertebrate eukaryotic Y-box proteins. The CSD of yps was purified and gel-shift analysis showed that this domain can interact with RNA. We predict that YPS would be an RNA-binding protein due to these results and the motifs which have been identified within the amino acid sequence.

MeSH Terms
Amino Acid Sequence Animals CCAAT-Enhancer-Binding Proteins Chromosomes/genetics Cloning, Molecular DNA-Binding Proteins/chemistry,genetics Drosophila Proteins Drosophila melanogaster/genetics Gene Expression Humans In Situ Hybridization Insect Proteins/genetics Molecular Sequence Data NFI Transcription Factors Nuclear Proteins Open Reading Frames RNA-Binding Proteins/chemistry,genetics Transcription Factors Y-Box-Binding Protein 1
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins Drosophila Proteins Insect Proteins NFI Transcription Factors Nuclear Proteins RNA-Binding Proteins Transcription Factors Y-Box-Binding Protein 1 YBX1 protein, human YPS protein, Drosophila
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Thieringer H A
Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA.
Singh K
Trivedi H
Inouye M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/25.23.4764
Published
1997-12-01
Pages
4764-70
Language
English
Region
England
NLM ID
0411011
PMCID
PMC147121
Subset
IM
Grants
NIGMS NIH HHS · GM19043 · United States
Databases
GENBANK
U49120
External Links
PubMed source DOI: 10.1093/nar/25.23.4764 PMC Full Text View journal details
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