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PMID: 9620847 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RNA-binding protein conserved in both microtubule- and microfilament-based RNA localization.

Genes & development ·Vol. 12 ·No. 11 ·1998-06-01 ·Pages 1593-8

Havin L, Git A, Elisha Z, Oberman F, Yaniv K, Schwartz SP, Standart N, Yisraeli JK

Abstract

Vg1 mRNA translocation to the vegetal cortex of Xenopus oocytes requires intact microtubules, and a 3' UTR cis-acting element (termed VLE), which also mediates sequence-specific binding of several proteins. One protein, the 69-kD Vg1 RBP, associates Vg1 RNA to microtubules in vitro. Here we show that Vg1 RBP-binding sites correlate with vegetal localization. Purification and cloning of Vg1 RBP revealed five RNA-binding motifs: four KH and one RRM domains. Surprisingly, Vg1 RBP is highly homologous to the zipcode binding protein implicated in the microfilament-mediated localization of beta actin mRNA in fibroblasts. These data support Vg1 RBP's direct role in vegetal localization and suggest the existence of a general, evolutionarily conserved mechanism for mRNA targeting.

MeSH Terms
Actin Cytoskeleton/metabolism Amino Acid Sequence Animals Binding Sites/genetics Female Glycoproteins/genetics,metabolism Microtubules/metabolism Molecular Sequence Data Oocytes/metabolism,ultrastructure Protein Binding RNA, Messenger/genetics,metabolism RNA-Binding Proteins/genetics,metabolism Sequence Alignment Transforming Growth Factor beta Xenopus Xenopus Proteins
Chemicals
GDF1 protein, Xenopus Glycoproteins RNA, Messenger RNA-Binding Proteins Transforming Growth Factor beta Xenopus Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Havin L
Department of Anatomy and Cell Biology, Hebrew University Medical School, Jerusalem 91120, Israel.
Git A
Elisha Z
Oberman F
Yaniv K
Schwartz S P
Standart N
Yisraeli J K
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.11.1593
Published
1998-06-01
Pages
1593-8
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316865
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.12.11.1593 PMC Full Text View journal details
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