Home LiteratureArticle Details
PMID: 10648791 Published · ppublish English Journal Article

Stp1p, Stp2p and Abf1p are involved in regulation of expression of the amino acid transporter gene BAP3 of Saccharomyces cerevisiae.

Nucleic acids research ·Vol. 28 ·No. 4 ·2000-02-15 ·Pages 974-81

de Boer M, Nielsen PS, Bebelman JP, Heerikhuizen H, Andersen HA, Planta RJ

Abstract

Expression of the BAP3 gene of Saccharomyces cerevisiae, encoding a branched chain amino acid permease, is induced in response to the availability of several naturally occurring amino acids in the medium. This induction is mediated via an upstream activating sequence (called UAS(aa)) in the BAP3 promoter, and dependent on Stp1p, a nuclear protein with zinc finger domains, suggesting that Stp1p is a transcription factor involved in BAP3 expression. In this paper, we show that Stp2p, a protein with considerable similarity to Stp1p, is also involved in the induction of BAP3 expression. To gain more insight into the roles of STP1 and STP2, we have overexpressed both Stp1p and Stp2p in yeast cells. Gel shift assays with the UAS(aa)as a probe show that the UAS(aa)can form two major complexes. One complex is dependent on Stp2p overexpression and the other is formed independently of STP1 or STP2, suggesting that the UAS(aa)is also bound by another factor. Here we show that the other factor is Abf1p, which binds specifically to the UAS(aa)of BAP3.

MeSH Terms
Amino Acid Transport Systems Artificial Gene Fusion Base Sequence Cloning, Molecular DNA Primers Escherichia coli/genetics Fungal Proteins/genetics,metabolism,physiology Gene Expression Regulation, Fungal/physiology Lac Operon Membrane Proteins/genetics Membrane Transport Proteins/genetics Polynucleotide Adenylyltransferase Protein Binding Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins
Chemicals
Amino Acid Transport Systems BAP3 protein, S cerevisiae DNA Primers Fungal Proteins Membrane Proteins Membrane Transport Proteins Saccharomyces cerevisiae Proteins PAP1 protein, S cerevisiae Polynucleotide Adenylyltransferase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
de Boer M
Department of Biochemistry and Molecular Biology, Faculty of Sciences, BioCentrum Amsterdam, Vrije Universiteit, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands.
Nielsen P S
Bebelman J P
Heerikhuizen H
Andersen H A
Planta R J
References (28)
28 references, click to expand
  1. Proteinase mutants of Saccharomyces cerevisiae.
    Genetics. 1977 Jan;85(1):23-33 PMID: 320092
  2. Amino acid signaling in Saccharomyces cerevisiae: a permease-like sensor of external amino acids and F-Box protein Grr1p are required for transcriptional induction of the AGP1 gene, which encodes a broad-specificity amino acid permease.
    Mol Cell Biol. 1999 Feb;19(2):989-1001 PMID: 9891035
  3. Isolation of a yeast gene involved in species-specific pre-tRNA processing.
    Mol Cell Biol. 1988 Dec;8(12):5140-9 PMID: 3072476
  4. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.
    Gene. 1988 Dec 30;74(2):527-34 PMID: 3073106
  5. Sequence, expression and mutational analysis of BAF1, a transcriptional activator and ARS1-binding protein of the yeast Saccharomyces cerevisiae.
    EMBO J. 1989 Dec 20;8(13):4265-72 PMID: 2686983
  6. Yeast general transcription factor GFI: sequence requirements for binding to DNA and evolutionary conservation.
    Nucleic Acids Res. 1990 May 11;18(9):2769-76 PMID: 2187179
  7. High efficiency transformation of Escherichia coli with plasmids.
    Gene. 1990 Nov 30;96(1):23-8 PMID: 2265755
  8. Regulation of transcription of the gene coding for peroxisomal 3-oxoacyl-CoA thiolase of Saccharomyces cerevisiae.
    Eur J Biochem. 1991 Aug 15;200(1):113-22 PMID: 1715273
  9. One-step transformation of yeast in stationary phase.
    Curr Genet. 1992 Jan;21(1):83-4 PMID: 1735128
  10. Role of multifunctional autonomously replicating sequence binding factor 1 in the initiation of DNA replication and transcriptional control in Saccharomyces cerevisiae.
    Mol Cell Biol. 1992 Mar;12(3):1064-77 PMID: 1545789
  11. STP1, a gene involved in pre-tRNA processing, encodes a nuclear protein containing zinc finger motifs.
    Mol Cell Biol. 1992 Jun;12(6):2633-43 PMID: 1588961
  12. ABF1 is a phosphoprotein and plays a role in carbon source control of COX6 transcription in Saccharomyces cerevisiae.
    Mol Cell Biol. 1992 Sep;12(9):4197-208 PMID: 1324416
  13. Comparison of the Saccharomyces cerevisiae G1 cyclins: Cln3 may be an upstream activator of Cln1, Cln2 and other cyclins.
    EMBO J. 1993 May;12(5):1955-68 PMID: 8387915
  14. A regulatory element in the CHA1 promoter which confers inducibility by serine and threonine on Saccharomyces cerevisiae genes.
    Mol Cell Biol. 1993 Dec;13(12):7604-11 PMID: 8246977
  15. Cloning and chromosomal organization of a gene encoding a putative amino-acid permease from Saccharomyces cerevisiae.
    Gene. 1994 May 27;143(1):129-33 PMID: 8200527
  16. Nucleosome positioning and modification: chromatin structures that potentiate transcription.
    Trends Biochem Sci. 1994 Jun;19(6):240-4 PMID: 8073501
  17. Two FK506 resistance-conferring genes in Saccharomyces cerevisiae, TAT1 and TAT2, encode amino acid permeases mediating tyrosine and tryptophan uptake.
    Mol Cell Biol. 1994 Oct;14(10):6597-606 PMID: 7523855
  18. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.
    Yeast. 1994 Dec;10(13):1793-808 PMID: 7747518
  19. Activation and repression of the yeast ARO3 gene by global transcription factors.
    Mol Microbiol. 1995 Jan;15(1):167-78 PMID: 7752892
  20. Structure-function analysis of the DNA binding domain of Saccharomyces cerevisiae ABF1.
    Nucleic Acids Res. 1995 Aug 11;23(15):2980-7 PMID: 7659521
  21. The Saccharomyces cerevisiae HSP12 gene is activated by the high-osmolarity glycerol pathway and negatively regulated by protein kinase A.
    Mol Cell Biol. 1995 Nov;15(11):6232-45 PMID: 7565776
  22. BAP2, a gene encoding a permease for branched-chain amino acids in Saccharomyces cerevisiae.
    Biochim Biophys Acta. 1995 Nov 30;1269(3):275-80 PMID: 7495881
  23. STP1, a gene involved in pre-tRNA processing in yeast, is important for amino-acid uptake and transcription of the permease gene BAP2.
    Curr Genet. 1997 Mar;31(3):241-7 PMID: 9065387
  24. Regulation of the yeast HIS7 gene by the global transcription factor Abf1p.
    Mol Gen Genet. 1997 Sep;256(2):136-46 PMID: 9349705
  25. The permease homologue Ssy1p controls the expression of amino acid and peptide transporter genes in Saccharomyces cerevisiae.
    Mol Microbiol. 1998 Feb;27(3):643-50 PMID: 9489675
  26. The Saccharomyces cerevisiae YCC5 (YCL025c) gene encodes an amino acid permease, Agp1, which transports asparagine and glutamine.
    J Bacteriol. 1998 May;180(9):2556-9 PMID: 9573211
  27. Regulation of expression of the amino acid transporter gene BAP3 in Saccharomyces cerevisiae.
    Mol Microbiol. 1998 Nov;30(3):603-13 PMID: 9822825
  28. Codon replacement in the PGK1 gene of Saccharomyces cerevisiae: experimental approach to study the role of biased codon usage in gene expression.
    Mol Cell Biol. 1987 Aug;7(8):2914-24 PMID: 2823108
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/28.4.974
Published
2000-02-15
Pages
974-81
Language
English
Region
England
NLM ID
0411011
PMCID
PMC102570
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/28.4.974 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2