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PMID: 10639466 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional conservation of the polysaccharide biosynthetic protein WbpM and its homologues in Pseudomonas aeruginosa and other medically significant bacteria.

Infection and immunity ·Vol. 68 ·No. 2 ·2000-02-00 ·Pages 931-6

Burrows LL, Urbanic RV, Lam JS

Abstract

WbpM is a highly conserved protein involved in synthesis of the O antigens of Pseudomonas aeruginosa. Homologues of this protein have been identified in a large number of bacteria, and they can be divided into two subfamilies: subfamily 1, including WbpM, contains large proteins ( approximately 600 amino acids), while subfamily 2, typified by HP0840 (FlaA1) of Helicobacter pylori, contains smaller proteins ( approximately 350 amino acids) homologous to the C termini of proteins in subfamily 1. Analysis of knockout mutants of wbpM in P. aeruginosa serotypes O3, O10, O15, and O17 showed that although all 20 serotypes of P. aeruginosa possess wbpM, it is not universally required for O-antigen biosynthesis. Homologous genes from Bordetella pertussis (wlbL), Staphylococcus aureus (cap8D), and H. pylori (flaA1) complemented a P. aeruginosa O5 wbpM mutant to various degrees. These conserved proteins may represent interesting targets for the design of inhibitors of bacterial exopolysaccharide biosynthesis.

MeSH Terms
Bacterial Proteins/physiology O Antigens/biosynthesis Pseudomonas aeruginosa/immunology
Chemicals
Bacterial Proteins O Antigens
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Burrows L L
The Center for Infection and Biomaterials Research, Toronto General Hospital, University of Toronto, Toronto, Ontario.
Urbanic R V
Lam J S
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.68.2.931-936.2000
Published
2000-02-00
Pages
931-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC97225
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.68.2.931-936.2000 PMC Full Text View journal details
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