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PMID: 10637315 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Association of mouse actin-binding protein 1 (mAbp1/SH3P7), an Src kinase target, with dynamic regions of the cortical actin cytoskeleton in response to Rac1 activation.

Molecular biology of the cell ·Vol. 11 ·No. 1 ·2000-01-00 ·Pages 393-412

Kessels MM, Engqvist-Goldstein AE, Drubin DG

Abstract

Yeast Abp1p is a cortical actin cytoskeleton protein implicated in cytoskeletal regulation, endocytosis, and cAMP-signaling. We have identified a gene encoding a mouse homologue of Abp1p, and it is identical to SH3P7, a protein shown recently to be a target of Src tyrosine kinases. Yeast and mouse Abp1p display the same domain structure including an N-terminal actin-depolymerizing factor homology domain and a C-terminal Src homology 3 domain. Using two independent actin-binding domains, mAbp1 binds to actin filaments with a 1:5 saturation stoichiometry. In stationary cells, mAbp1 colocalizes with cortical F-actin in fibroblast protrusions that represent sites of cellular growth. mAbp1 appears at the actin-rich leading edge of migrating cells. Growth factors cause mAbp1 to rapidly accumulate in lamellipodia. This response can be mimicked by expression of dominant-positive Rac1. mAbp1 recruitment appears to be dependent on de novo actin polymerization and occurs specifically at sites enriched for the Arp2/3 complex. mAbp1 is a newly identified cytoskeletal protein in mice and may serve as a signal-responsive link between the dynamic cortical actin cytoskeleton and regions of membrane dynamics.

MeSH Terms
3T3 Cells Actins/metabolism Amino Acid Sequence Animals Binding Sites COS Cells Cell Division Cell Line Cell Movement Cytoskeleton Endocytosis Humans Intracellular Fluid/metabolism Mice Microfilament Proteins/genetics,metabolism Molecular Sequence Data Platelet-Derived Growth Factor/pharmacology Polymers RNA, Messenger Sequence Analysis Staining and Labeling Tetradecanoylphorbol Acetate/pharmacology rac1 GTP-Binding Protein/metabolism src Homology Domains src-Family Kinases/metabolism
Chemicals
Actins DBNL protein, human Dbnl protein, mouse Microfilament Proteins Platelet-Derived Growth Factor Polymers RNA, Messenger src-Family Kinases rac1 GTP-Binding Protein Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kessels M M
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720-3202, USA.
Engqvist-Goldstein A E
Drubin D G
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.11.1.393
Published
2000-01-00
Pages
393-412
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC14781
Subset
IM
Grants
NIDDK NIH HHS · P01 DK032094 · United States
NIGMS NIH HHS · R01 GM050399 · United States
NIDDK NIH HHS · DK-32094 · United States
NIGMS NIH HHS · GM-50399 · United States
External Links
PubMed source DOI: 10.1091/mbc.11.1.393 PMC Full Text View journal details
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