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PMID: 10631247 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Villin-like actin-binding proteins are expressed ubiquitously in Arabidopsis.

Plant physiology ·Vol. 122 ·No. 1 ·2000-01-00 ·Pages 35-48

Klahre U, Friederich E, Kost B, Louvard D, Chua NH

Abstract

In an attempt to elucidate the biological function of villin-like actin-binding proteins in plants we have cloned several genes encoding Arabidopsis proteins with high homology to animal villin. We found that Arabidopsis contains at least four villin-like genes (AtVLNs) encoding four different VLN isoforms. Two AtVLN isoforms are more closely related to mammalian villin in their primary structure and are also antigenically related, whereas the other two contain significant changes in the C-terminal headpiece domain. RNA and promoter/beta-glucuronidase expression studies demonstrated that AtVLN genes are expressed in all organs, with elevated expression levels in certain types of cells. These results suggest that AtVLNs have less-specialized functions than mammalian villin, which is found only in the microvilli of brush border cells. Immunoblot experiments using a monoclonal antibody against pig villin showed that AtVLNs are widely distributed in a variety of plant tissues. Green fluorescent protein fused to full-length AtVLN and individual AtVLN headpiece domains can bind to both animal and plant actin filaments in vivo.

MeSH Terms
Amino Acid Sequence Animals Arabidopsis/genetics,metabolism Carrier Proteins/genetics,metabolism Chlorocebus aethiops Microfilament Proteins/genetics,metabolism Microscopy, Confocal Molecular Sequence Data Plant Proteins/genetics,metabolism Plants, Toxic Sequence Homology, Amino Acid Tobacco/cytology,metabolism Vero Cells
Chemicals
Carrier Proteins Microfilament Proteins Plant Proteins villin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Klahre U
Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.
Friederich E
Kost B
Louvard D
Chua N H
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.122.1.35
Published
2000-01-00
Pages
35-48
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC58842
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.122.1.35 PMC Full Text View journal details
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