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PMID: 1961750 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure of the human villin gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 23 ·1991-12-01 ·Pages 10811-5

Pringault E, Robine S, Louvard D

Abstract

We have isolated and characterized the complete human villin gene. The villin gene is located on chromosome 2q35-36 in humans and on chromosome 1 in mice. Villin belongs to a family of calcium-regulated actin-binding proteins that share structural and functional homologies. The villin gene is expressed mainly in cells that develop a brush border, such as mucosal cells of the small and large intestine and epithelial cells of the kidney proximal tubules. Villin gene expression is strictly regulated during adult life and embryonic development in the digestive and urogenital tracts and, thus, may be used as a marker of the digestive and renal cell lineages. The human villin gene has one copy per haploid genome, encompasses about 25 kilobases, and contains 19 exons. Analysis of the structural organization of this gene shows that the two mRNAs that encode villin in humans arise by alternative choice of one of the two polyadenylylation signals located within the last exon. The overall organization of the exons reflects the gene duplication event from which this family of actin-binding proteins originated.

MeSH Terms
Actins/genetics Animals Base Sequence Calcium-Binding Proteins/genetics Carrier Proteins/genetics Chromosome Mapping Chromosomes, Human, Pair 2 DNA/genetics,isolation & purification Exons Genomic Library Humans Introns Lymphocytes/physiology Mice Microfilament Proteins/genetics Molecular Sequence Data RNA Splicing RNA, Messenger/genetics Transcription, Genetic
Chemicals
Actins Calcium-Binding Proteins Carrier Proteins Microfilament Proteins RNA, Messenger villin DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pringault E
Département de Biologie Moléculaire, Institut Pasteur, Paris, France.
Robine S
Louvard D
References (22)
22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.23.10811
Published
1991-12-01
Pages
10811-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53021
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.23.10811 PMC Full Text View journal details
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