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PMID: 10629208 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The HTR1 gene is a dominant negative mutant allele of MTH1 and blocks Snf3- and Rgt2-dependent glucose signaling in yeast.

Journal of bacteriology ·Vol. 182 ·No. 2 ·2000-01-00 ·Pages 540-2

Schulte F, Wieczorke R, Hollenberg CP, Boles E

Abstract

Saccharomyces cerevisiae HTR1 mutants are severely impaired in the uptake of glucose. We have cloned dominant HTR1 mutant alleles and show that they encode mutant forms of the Mth1 protein. Mth1 is shown to be involved in carbon source-dependent regulation of its own, invertase and hexose transporter gene expression. The mutant forms block the transduction of the Snf3- and Rgt2-mediated glucose signals upstream of the Rgt1 transcriptional regulator.

MeSH Terms
Adaptor Proteins, Signal Transducing Alleles Cell Cycle Proteins Cloning, Molecular Fungal Proteins/genetics Gene Expression Regulation, Fungal Glucose/physiology Glycoside Hydrolases/metabolism Membrane Proteins/physiology Monosaccharide Transport Proteins/genetics,physiology Mutation RNA-Binding Proteins Repressor Proteins Saccharomyces cerevisiae/genetics,physiology Saccharomyces cerevisiae Proteins Signal Transduction/genetics beta-Fructofuranosidase
Chemicals
Adaptor Proteins, Signal Transducing Cell Cycle Proteins Fungal Proteins MPT5 protein, S cerevisiae MTH1 protein, S cerevisiae Membrane Proteins Monosaccharide Transport Proteins RGT2 protein, S cerevisiae RNA-Binding Proteins Repressor Proteins SNF3 protein, S cerevisiae Saccharomyces cerevisiae Proteins Glycoside Hydrolases beta-Fructofuranosidase Glucose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schulte F
Institut für Mikrobiologie, Heinrich-Heine-Universität, D-40225 Düsseldorf, Germany.
Wieczorke R
Hollenberg C P
Boles E
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.2.540-542.2000
Published
2000-01-00
Pages
540-2
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC94311
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.182.2.540-542.2000 PMC Full Text View journal details
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