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PMID: 8114728 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Dosage-dependent modulation of glucose repression by MSN3 (STD1) in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 14 ·No. 3 ·1994-03-00 ·Pages 1972-8

Hubbard EJ, Jiang R, Carlson M

Abstract

The SNF1 protein kinase of Saccharomyces cerevisiae is required to relieve glucose repression of transcription. To identify components of the SNF1 pathway, we isolated multicopy suppressors of defects caused by loss of SNF4, an activator of the SNF1 kinase. Increased dosage of the MSN3 gene restored invertase expression in snf4 mutants and also relieved glucose repression in the wild type. Deletion of MSN3 caused no substantial phenotype, and we identified a homolog, MTH1, encoding a protein 61% identical to MSN3. Both are also homologous to chicken fimbrin, human plastin, and yeast SAC6 over a 43-residue region. Deletion of MSN3 and MTH1 together impaired derepression of invertase in response to glucose limitation. Finally, MSN3 physically interacts with the SNF1 protein kinase, as assayed by a two-hybrid system and by in vitro binding studies. MSN3 is the same gene as STD1, a multicopy suppressor of defects caused by overexpression of the C terminus of TATA-binding protein (R. W. Ganster, W. Shen, and M. C. Schmidt, Mol. Cell. Biol. 13:3650-3659, 1993). Taken together, these data suggest that MSN3 modulates the regulatory response to glucose and may couple the SNF1 pathway to transcription.

Related Genes
MSN3 MTH1 P6STD1 SAC6 SNF1 SNF4
MeSH Terms
Adaptor Proteins, Signal Transducing Amino Acid Sequence Cloning, Molecular DNA Primers/chemistry Fibrin/chemistry Fungal Proteins/chemistry,genetics,physiology Gene Expression Regulation, Fungal Genes, Fungal Glucose/metabolism Intracellular Signaling Peptides and Proteins Membrane Glycoproteins Membrane Proteins Microfilament Proteins Molecular Sequence Data Mutagenesis, Insertional Phosphoproteins/chemistry Protein Serine-Threonine Kinases/metabolism RNA, Messenger/genetics Recombinant Fusion Proteins Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
Adaptor Proteins, Signal Transducing DNA Primers Fungal Proteins Intracellular Signaling Peptides and Proteins MTH1 protein, S cerevisiae Membrane Glycoproteins Membrane Proteins Microfilament Proteins Phosphoproteins RNA, Messenger Recombinant Fusion Proteins STD1 protein, S cerevisiae Saccharomyces cerevisiae Proteins plastin Fibrin SNF1-related protein kinases Protein Serine-Threonine Kinases Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hubbard E J
Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, New York, New York 10032.
Jiang R
Carlson M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.3.1972-1978.1994
Published
1994-03-00
Pages
1972-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358556
Subset
IM
Grants
NIGMS NIH HHS · GM34095 · United States
Databases
GENBANK
L21932, L21933
External Links
PubMed source DOI: 10.1128/mcb.14.3.1972-1978.1994 PMC Full Text View journal details
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