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PMID: 10548544 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA-binding activity of the transcription factor upstream stimulatory factor 1 (USF-1) is regulated by cyclin-dependent phosphorylation.

The Biochemical journal ·Vol. 344 Pt 1 ·1999-11-15 ·Pages 145-52

Cheung E, Mayr P, Coda-Zabetta F, Woodman PG, Boam DS

Abstract

The ubiquitous transcription factor upstream stimulatory factor (USF) 1 is a member of the bzHLH (leucine zipper-basic-helix-loop-helix) family, which is structurally related to the Myc family of proteins. It plays a role in the regulation of many genes, including the cyclin B1 gene, which is active during the G2/M and M phases of the cell cycle and may also play a role in the regulation of cellular proliferation. We show that the affinity of recombinant USF-1 for DNA is greatly increased by treatment with active cyclin A2-p34(cdc2) or cyclin B1-p34(cdc2) complexes and that its interaction with DNA is dependent on p34(cdc2)-mediated phosphorylation. We have localized the phosphorylation site(s) to a region that lies outside the minimal DNA-binding domain but overlaps with the previously identified USF-specific region. Deletion studies of USF-1 suggest that amino acids 143-197 regulate DNA-binding activity in a phosphorylation-dependent manner.

MeSH Terms
Animals Base Sequence Binding Sites CDC2 Protein Kinase/metabolism Cell Cycle Cyclins/genetics,metabolism DNA/metabolism DNA Probes/genetics DNA-Binding Proteins Female HeLa Cells Humans In Vitro Techniques Mitosis Oocytes/metabolism Phosphorylation Recombinant Proteins/genetics,metabolism Transcription Factors/genetics,metabolism Upstream Stimulatory Factors Xenopus
Chemicals
Cyclins DNA Probes DNA-Binding Proteins Recombinant Proteins Transcription Factors USF1 protein, human Upstream Stimulatory Factors DNA CDC2 Protein Kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cheung E
School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford Road, Manchester M13 9PT, U.K.
Mayr P
Coda-Zabetta F
Woodman P G
Boam D S
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Published
1999-11-15
Pages
145-52
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1220624
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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