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PMID: 1922036 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Recombinant 43-kDa USF binds to DNA and activates transcription in a manner indistinguishable from that of natural 43/44-kDa USF.

Molecular and cellular biology ·Vol. 11 ·No. 10 ·1991-10-00 ·Pages 5125-36

Pognonec P, Roeder RG

Abstract

USF is a cellular factor involved in the transcriptional regulation of several cellular and viral promoters. Purified USF from HeLa cells (HeLa USF) consists of 43- and 44-kDa polypeptides which show independent binding to a specific DNA element. A cDNA encoding the 43-kDa species has been previously cloned. We show here that the purified form of bacterially expressed 43-kDa USF (i) exists in solution as a dimer whose formation is greatly favored under reducing conditions, (ii) binds to its cognate DNA sequence in a manner indistinguishable from that of HeLa USF, and (iii) is as efficient as HeLa USF in stimulating transcription from target promoters in a reconstituted cell-free system. Additional data indicate that the 44-kDa component of HeLa USF is immunologically unrelated to the 43-kDa polypeptide but is associated with it in HeLa cell extracts. These results suggest that the 43-kDa component possesses an intrinsic DNA binding and transcriptional activation potential and that the 44-kDa USF component of the natural USF complex may have some regulatory role.

MeSH Terms
Base Sequence Blotting, Western Cell-Free System DNA/metabolism DNA-Binding Proteins HeLa Cells Humans Macromolecular Substances Molecular Sequence Data Promoter Regions, Genetic/physiology Recombinant Proteins/metabolism T-Phages/genetics Transcription Factors/metabolism Transcription, Genetic/physiology Upstream Stimulatory Factors
Chemicals
DNA-Binding Proteins Macromolecular Substances Recombinant Proteins Transcription Factors USF1 protein, human Upstream Stimulatory Factors DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pognonec P
Rockefeller University, New York, New York 10021-6399.
Roeder R G
References (47)
47 references, click to expand
  1. Factors responsible for the higher transcriptional activity of extracts of adenovirus-infected cells fractionate with the TATA box transcription factor.
    Mol Cell Biol. 1988 Apr;8(4):1765-74 PMID: 2967913
  2. Transcriptional regulator of oxidative stress-inducible genes: direct activation by oxidation.
    Science. 1990 Apr 13;248(4952):189-94 PMID: 2183352
  3. Multiple forms of the human gene-specific transcription factor USF. II. DNA binding properties and transcriptional activity of the purified HeLa USF.
    J Biol Chem. 1988 Aug 25;263(24):11994-2001 PMID: 3403559
  4. Physical analysis of transcription preinitiation complex assembly on a class II gene promoter.
    Science. 1988 Sep 9;241(4871):1335-8 PMID: 3413495
  5. I kappa B: a specific inhibitor of the NF-kappa B transcription factor.
    Science. 1988 Oct 28;242(4878):540-6 PMID: 3140380
  6. Direct interaction between fos and jun nuclear oncoproteins: role of the 'leucine zipper' domain.
    Nature. 1988 Dec 15;336(6200):692-5 PMID: 3143919
  7. Multiple factors required for accurate initiation of transcription by purified RNA polymerase II.
    J Biol Chem. 1980 Dec 25;255(24):11992-6 PMID: 7440580
  8. DNA sequence required for initiation of transcription in vitro from the major late promoter of adenovirus 2.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):820-4 PMID: 6262775
  9. Sequences upstream from the T-A-T-A box are required in vivo and in vitro for efficient transcription from the adenovirus serotype 2 major late promoter.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7132-6 PMID: 6961401
  10. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  11. Generation and functional analyses for base-substitution mutants of the adenovirus 2 major late promoter.
    Nucleic Acids Res. 1984 Dec 21;12(24):9309-21 PMID: 6514578
  12. Assembly of the mitochondrial membrane system. CBP6, a yeast nuclear gene necessary for synthesis of cytochrome b.
    J Biol Chem. 1985 Feb 10;260(3):1513-20 PMID: 2981859
  13. Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4394-8 PMID: 3925456
  14. Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.
    Cell. 1985 Nov;43(1):165-75 PMID: 4075392
  15. An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter.
    Cell. 1985 Dec;43(2 Pt 1):439-48 PMID: 4075400
  16. Specific interaction between a transcription factor and the upstream element of the adenovirus-2 major late promoter.
    EMBO J. 1985 Dec 16;4(13A):3563-70 PMID: 4092688
  17. In vivo identification of sequence elements required for normal function of the adenovirus major late transcriptional control region.
    Nucleic Acids Res. 1986 Aug 11;14(15):6327-35 PMID: 2944077
  18. Purification of a factor specific for the upstream element of the adenovirus-2 major late promoter.
    EMBO J. 1986 Oct;5(10):2577-84 PMID: 3023048
  19. A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor.
    Mol Cell Biol. 1986 Dec;6(12):4723-33 PMID: 3796614
  20. Adenovirus promoters and E1A transactivation.
    Annu Rev Genet. 1986;20:45-79 PMID: 3028247
  21. The adenovirus major late transcription factor activates the rat gamma-fibrinogen promoter.
    Science. 1987 Oct 30;238(4827):684-8 PMID: 3672119
  22. Identification and purification of a human lymphoid-specific octamer-binding protein (OTF-2) that activates transcription of an immunoglobulin promoter in vitro.
    Cell. 1987 Dec 4;51(5):783-93 PMID: 3119226
  23. The major late transcription factor binds to and activates the mouse metallothionein I promoter.
    Genes Dev. 1987 Nov;1(9):973-80 PMID: 3428603
  24. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Methods Enzymol. 1987;154:367-82 PMID: 3323813
  25. The adenovirus tripartite leader may eliminate the requirement for cap-binding protein complex during translation initiation.
    J Virol. 1988 Jun;62(6):2059-66 PMID: 2835510
  26. Five intermediate complexes in transcription initiation by RNA polymerase II.
    Cell. 1989 Feb 24;56(4):549-61 PMID: 2917366
  27. A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins.
    Cell. 1989 Mar 10;56(5):777-83 PMID: 2493990
  28. The DNA binding domain of the rat liver nuclear protein C/EBP is bipartite.
    Science. 1989 Mar 31;243(4899):1681-8 PMID: 2494700
  29. The "initiator" as a transcription control element.
    Cell. 1989 Apr 7;57(1):103-13 PMID: 2467742
  30. Fos and Jun bind cooperatively to the AP-1 site: reconstitution in vitro.
    Genes Dev. 1988 Dec;2(12B):1687-99 PMID: 2467839
  31. The c-ets-1 protein is chromatin associated and binds to DNA in vitro.
    Oncogene. 1989 Jun;4(6):691-7 PMID: 2660071
  32. Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence.
    Cell. 1989 Aug 11;58(3):537-44 PMID: 2503252
  33. Replication-dependent activation of the adenovirus major late promoter is mediated by the increased binding of a transcription factor to sequences in the first intron.
    J Virol. 1989 Dec;63(12):5124-32 PMID: 2585599
  34. Dimers, leucine zippers and DNA-binding domains.
    Trends Genet. 1990 Feb;6(2):36-40 PMID: 2186528
  35. TFE3: a helix-loop-helix protein that activates transcription through the immunoglobulin enhancer muE3 motif.
    Genes Dev. 1990 Feb;4(2):167-79 PMID: 2338243
  36. The mammalian upstream element factor recognizes two sites in the adenovirus type 2 IVa2-major late promoter intergenic region and stimulates both promoters.
    J Virol. 1990 Jul;64(7):3199-206 PMID: 2352321
  37. A helix-loop-helix protein related to the immunoglobulin E box-binding proteins.
    Mol Cell Biol. 1990 Aug;10(8):4384-8 PMID: 2115126
  38. Use of T7 RNA polymerase to direct expression of cloned genes.
    Methods Enzymol. 1990;185:60-89 PMID: 2199796
  39. Redox regulation of fos and jun DNA-binding activity in vitro.
    Science. 1990 Sep 7;249(4973):1157-61 PMID: 2118682
  40. Nuclear localization of c-Fos, but not v-Fos proteins, is controlled by extracellular signals.
    Cell. 1990 Oct 19;63(2):341-51 PMID: 2119889
  41. The adenovirus major late transcription factor USF is a member of the helix-loop-helix group of regulatory proteins and binds to DNA as a dimer.
    Genes Dev. 1990 Oct;4(10):1730-40 PMID: 2249772
  42. Transcription factor AP-4 contains multiple dimerization domains that regulate dimer specificity.
    Genes Dev. 1990 Oct;4(10):1741-52 PMID: 2123466
  43. The Xenopus B1 factor is closely related to the mammalian activator USF and is implicated in the developmental regulation of TFIIIA gene expression.
    Mol Cell Biol. 1991 Jan;11(1):412-24 PMID: 1986236
  44. Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc.
    Science. 1991 Mar 8;251(4998):1211-7 PMID: 2006410
  45. Repression of HIV-1 transcription by a cellular protein.
    Science. 1991 Mar 22;251(5000):1476-9 PMID: 2006421
  46. High-level transcription from the adenovirus major late promoter requires downstream binding sites for late-phase-specific factors.
    J Virol. 1990 Jan;64(1):51-60 PMID: 2293670
  47. Multiple forms of the human gene-specific transcription factor USF. I. Complete purification and identification of USF from HeLa cell nuclei.
    J Biol Chem. 1988 Aug 25;263(24):11985-93 PMID: 3403558
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.10.5125-5136.1991
Published
1991-10-00
Pages
5125-36
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361528
Subset
IM
Grants
NIAID NIH HHS · AI27397 · United States
NCI NIH HHS · CA42567 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.10.5125-5136.1991 PMC Full Text View journal details
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