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PMID: 10377344 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The native rat olfactory cyclic nucleotide-gated channel is composed of three distinct subunits.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 19 ·No. 13 ·1999-07-01 ·Pages 5332-47

Bönigk W, Bradley J, Müller F, Sesti F, Boekhoff I, Ronnett GV, Kaupp UB, Frings S

Abstract

Cyclic nucleotide-gated (CNG) channels play central roles in visual and olfactory signal transduction. In the retina, rod photoreceptors express the subunits CNCalpha1 and CNCbeta1a. In cone photoreceptors, only CNCalpha2 expression has been demonstrated so far. Rat olfactory sensory neurons (OSNs) express two homologous subunits, here designated CNCalpha3 and CNCalpha4. This paper describes the characterization of CNCbeta1b, a third subunit expressed in OSNs and establishes it as a component of the native channel. CNCbeta1b is an alternate splice form of the rod photoreceptor CNCbeta1a subunit. Analysis of mRNA and protein expression together suggest co-expression of all three subunits in sensory cilia of OSNs. From single-channel analyses of native rat olfactory channels and of channels expressed heterologously from all possible combinations of the CNCalpha3, -alpha4, and -beta1b subunits, we conclude that the native CNG channel in OSNs is composed of all three subunits. Thus, CNG channels in both rod photoreceptors and olfactory sensory neurons result from coassembly of specific alpha subunits with various forms of an alternatively spliced beta subunit.

MeSH Terms
Alternative Splicing Amino Acid Sequence Animals Cell Line Cilia/metabolism Cloning, Molecular Cyclic AMP/pharmacology Cyclic Nucleotide-Gated Cation Channels Epithelium/metabolism Gene Expression Humans Ion Channel Gating/drug effects Ion Channels/chemistry,genetics,metabolism,physiology Molecular Sequence Data Neurons, Afferent/metabolism Olfactory Bulb/cytology Patch-Clamp Techniques Potassium/metabolism RNA, Messenger/analysis,genetics,metabolism Rats Rats, Sprague-Dawley Sodium/metabolism
Chemicals
Cyclic Nucleotide-Gated Cation Channels Ion Channels RNA, Messenger olfactory cyclic-nucleotide-gated channel 2 Sodium Cyclic AMP Potassium
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Bönigk W
Forschungszentrum Jülich, Institut für Biologische Informationsverarbeitung, 52425 Jülich, Germany.
Bradley J
Müller F
Sesti F
Boekhoff I
Ronnett G V
Kaupp U B
Frings S
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1999-07-01
Pages
5332-47
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6782342
Subset
IM
Grants
NIDCD NIH HHS · R01DC02979 · United States
Databases
GENBANK
AF068572
External Links
PubMed source PMC Full Text View journal details
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