Chromosome-membrane association in Bacillus subtilis. IV. Further purification of DNA-membrane complex by using a combination of centrifugation and electrophoresis.

Journal of bacteriology ·Vol. 136 ·No. 2 ·1978-11-00 ·Pages 799-802

Toyoda H, Yamaguchi K, Yoshikawa H

Abstract

We have developed a simple procedure to purify a DNA-membrane complex from Bacillus subtilis by using a combination of centrifugation and electrophoresis. Several unique proteins were detected in the purified complex.

MeSH Terms

Bacillus subtilis/ultrastructure Bacterial Proteins/analysis Cell Fractionation/methods Cell Membrane Centrifugation Chromosomes, Bacterial DNA, Bacterial/isolation & purification Electrophoresis Membrane Proteins/analysis

Chemicals

Bacterial Proteins DNA, Bacterial Membrane Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Toyoda H

Yamaguchi K

Yoshikawa H

References (17)

17 references, click to expand

Deoxyribonucleic acid-envelope complexes from Escherichia coli. A complex-specific protein and its possible function for the stability of the complex.
J Cell Biol. 1975 Nov;67(2PT.1):444-60 PMID: 1104640
A DEOXYRIBONUCLEIC ACID-PROTEIN COMPLEX HAVING DNA-POLYMERASE AND RNA-POLYMERASE ACTIVITIES IN CELL-FREE EXTRACTS OF ESCHERICHIA COLI.
Biochim Biophys Acta. 1964 Sep 11;91:36-45 PMID: 14227277
Altered accumulation of a membrane protein unique to a membrane-deoxyribonucleic acid complex in a dna initiation mutant of Bacillus subtilis.
J Bacteriol. 1977 Jun;130(3):1224-33 PMID: 405373
Topography of chromosome membrane junction in Bacillus subtilis.
Nat New Biol. 1973 Aug 15;244(137):204-6 PMID: 4199319
Maturation of the head of bacteriophage T4. I. DNA packaging events.
J Mol Biol. 1973 Nov 15;80(4):575-99 PMID: 4204102
Isolation of DNA-membrane complex in Bacillus subtilis.
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4787-91 PMID: 4216023
Deoxyribonucleic acid-envelope complexes isolated from Escherichia coli by free-flow electrophoresis: biochemical and electron microscope characterization.
J Bacteriol. 1974 May;118(2):646-53 PMID: 4597456
Evidence for stable attachment of DNA to membrane at the replication origin of Escherichia coli.
Biochem Biophys Res Commun. 1970 Oct 9;41(1):157-62 PMID: 4918012
Changes of membrane proteins and their relation to deoxyribonucleic acid synthesis and cell division of Escherichia coli.
J Biol Chem. 1970 Nov 10;245(21):5813-9 PMID: 4919490
Isolation and characterization of the DNA replication complex from Escherichia coli.
J Mol Biol. 1970 Sep 14;52(2):301-22 PMID: 4922216
On the process of cellular division in Escherichia coli. IV. Altered protein composition and turnover of the membranes of thermosensitive mutants defective in chromosomal replication.
J Mol Biol. 1971 Mar 28;56(3):475-90 PMID: 4929576
Membrane attachment of the chromosome replication origin in Bacillus subtilis.
Cold Spring Harb Symp Quant Biol. 1968;33:695-705 PMID: 4977758
Association between the chromosome and the cytoplasmic membrane in Bacillus subtilis.
Biochem Biophys Res Commun. 1969 May 8;35(3):354-62 PMID: 4977972
Association of the Bacillus subtilis chromosome with the cell membrane: resolution of free and bound deoxyribonucleic acid on renografin gradients.
J Bacteriol. 1970 Nov;104(2):839-50 PMID: 4992373
Chromosome-membrane association in Bacillus subtilis. I. DNA release from membrane fraction.
Biochem Biophys Res Commun. 1971 Sep 17;44(6):1559-65 PMID: 5003692
Evidence of the involvement of an outer membrane protein in DNA initiation.
J Biol Chem. 1976 Jun 10;251(11):3470-9 PMID: 776971
Association of the replication terminus of the Bacillus subtilis chromosome to the cell membrane.
J Bacteriol. 1975 Nov;124(2):1030-3 PMID: 810475

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.136.2.799-802.1978

Published

1978-11-00

Pages

799-802

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC218608

Subset

External Links

PubMed source DOI: 10.1128/jb.136.2.799-802.1978 PMC Full Text View journal details

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Chromosome-membrane association in Bacillus subtilis. IV. Further purification of DNA-membrane complex by using a combination of centrifugation and electrophoresis.

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