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PMID: 10094707 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mutational analysis of the vacA promoter provides insight into gene transcription in Helicobacter pylori.

Journal of bacteriology ·Vol. 181 ·No. 7 ·1999-04-00 ·Pages 2261-6

Forsyth MH, Cover TL

Abstract

Analysis of 12 Helicobacter pylori promoters indicates the existence of a consensus -10 hexamer (TAtaaT) but little conservation of -35 sequences. In this study, mutations in either the H. pylori vacA -10 region or the -35 region resulted in decreased vacA transcription and suggested that an extended -10 motif is utilized. Thus, despite the lack of a -35 consensus sequence for H. pylori promoters, the -35 region plays a functional role in vacA transcription.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Bacterial Toxins/genetics Base Sequence Consensus Sequence DNA, Bacterial DNA-Directed RNA Polymerases/genetics Gene Expression Regulation, Bacterial Genes, Bacterial Helicobacter pylori/genetics Molecular Sequence Data Mutagenesis Promoter Regions, Genetic Sigma Factor/genetics Transcription, Genetic
Chemicals
Bacterial Proteins Bacterial Toxins DNA, Bacterial Sigma Factor VacA protein, Helicobacter pylori RNA polymerase sigma 70 DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Forsyth M H
Departments of Medicine and Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2605, USA.
Cover T L
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.7.2261-2266.1999
Published
1999-04-00
Pages
2261-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC93642
Subset
IM
Grants
NCI NIH HHS · P30 CA068485 · United States
NIAID NIH HHS · R01 AI039657 · United States
NIAID NIH HHS · AI 39657 · United States
NCI NIH HHS · CA68485 · United States
External Links
PubMed source DOI: 10.1128/JB.181.7.2261-2266.1999 PMC Full Text View journal details
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