Kinetics of rat liver glucokinase. Co-operative interactions with glucose at physiologically significant concentrations.

The Biochemical journal ·Vol. 159 ·No. 1 ·1976-10-01 ·Pages 7-14

Storer AC, Cornish-Bowden A

Abstract

The kinetics of glucokinase from rat liver were studied over wide ranges of glucose and MgATP2- concentrations. The initial rate shows a co-operative dependence on the glucose concentration, with Hill coefficients in the range 1.2-1.5. The degree of glucose co-operativity increases with the MgATP2- concentration, but no co-operativity was detected for the dependence of the rate on the MgATP2- concentration. The effects observed occur at physiologically reasonable concentrations of glucose and MgATP2- and are consistent with the presumed function of glucokinase in maintaining a constant concentration of glucose in the blood.

MeSH Terms

Adenosine Triphosphate/analysis Animals Blood Glucose Glucokinase/metabolism Glucose/analysis,metabolism Kinetics Liver/enzymology Magnesium/analysis Rats

Chemicals

Blood Glucose Adenosine Triphosphate Glucokinase Magnesium Glucose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Storer A C

Cornish-Bowden A

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27 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1590007

Published

1976-10-01

Pages

7-14

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1164031

Subset

External Links

PubMed source DOI: 10.1042/bj1590007 PMC Full Text View journal details

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Kinetics of rat liver glucokinase. Co-operative interactions with glucose at physiologically significant concentrations.

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