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PMID: 9990016 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interactions between human cyclin T, Tat, and the transactivation response element (TAR) are disrupted by a cysteine to tyrosine substitution found in mouse cyclin T.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 4 ·1999-02-16 ·Pages 1285-90

Fujinaga K, Taube R, Wimmer J, Cujec TP, Peterlin BM

Abstract

The transcriptional transactivator Tat from HIV binds to the transactivation response element (TAR) RNA to increase rates of elongation of viral transcription. Human cyclin T supports these interactions between Tat and TAR. In this study, we report the sequence of mouse cyclin T and identify the residues from positions 1 to 281 in human cyclin T that bind to Tat and TAR. Mouse cyclin T binds to Tat weakly and is unable to facilitate interactions between Tat and TAR. Reciprocal exchanges of the cysteine and tyrosine at position 261 in human and mouse cyclin T proteins also render human cyclin T inactive and mouse cyclin T active. These findings reveal the molecular basis for the restriction of Tat transactivation in rodent cells.

MeSH Terms

Amino Acid Sequence Amino Acid Substitution Animals CHO Cells Chloramphenicol O-Acetyltransferase/metabolism Cricetinae Cyclin T Cyclins/chemistry,genetics,metabolism Cysteine Gene Products, tat/metabolism HIV Long Terminal Repeat/physiology Humans Mice Molecular Sequence Data Mutagenesis, Site-Directed Recombinant Fusion Proteins/metabolism Recombinant Proteins/chemistry,metabolism Sequence Alignment Sequence Homology, Amino Acid Tyrosine

Chemicals

CCNT1 protein, human Ccnt1 protein, mouse Cyclin T Cyclins Gene Products, tat Recombinant Fusion Proteins Recombinant Proteins Tyrosine Chloramphenicol O-Acetyltransferase Cysteine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Fujinaga K

Howard Hughes Medical Institute, Departments of Medicine, Microbiology, and Immunology, University of California, San Francisco, CA 94143-0703, USA.

Taube R

Wimmer J

Cujec T P

Peterlin B M

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.96.4.1285

Published

1999-02-16

Pages

1285-90

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC15455

Subset

Databases

GENBANK

AF113951

External Links

PubMed source DOI: 10.1073/pnas.96.4.1285 PMC Full Text View journal details

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Interactions between human cyclin T, Tat, and the transactivation response element (TAR) are disrupted by a cysteine to tyrosine substitution found in mouse cyclin T.

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