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PMID: 9971746 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

delta-catenin, an adhesive junction-associated protein which promotes cell scattering.

The Journal of cell biology ·Vol. 144 ·No. 3 ·1999-02-08 ·Pages 519-32

Lu Q, Paredes M, Medina M, Zhou J, Cavallo R, Peifer M, Orecchio L, Kosik KS

Abstract

The classical adherens junction that holds epithelial cells together consists of a protein complex in which members of the cadherin family linked to various catenins are the principal components. delta-catenin is a mammalian brain protein in the Armadillo repeat superfamily with sequence similarity to the adherens junction protein p120(ctn). We found that delta-catenin can be immunoprecipitated as a complex with other components of the adherens junction, including cadherin and beta-catenin, from transfected cells and brain. The interaction with cadherin involves direct contact within the highly conserved juxtamembrane region of the COOH terminus, where p120(ctn) also binds. In developing mouse brain, staining with delta-catenin antibodies is prominent towards the apical boundary of the neuroepithelial cells in the ventricular zone. When transfected into Madin-Darby canine kidney (MDCK) epithelial cells delta-catenin colocalized with cadherin, p120(ctn), and beta-catenin. The Arm domain alone was sufficient for achieving localization and coimmunoprecipitation with cadherin. The ectopic expression of delta-catenin in MDCK cells altered their morphology, induced the elaboration of lamellipodia, interfered with monolayer formation, and increased scattering in response to hepatocyte growth factor treatment. We propose that delta-catenin can regulate adhesion molecules to implement the organization of large cellular arrays necessary for tissue morphogenesis.

MeSH Terms
Animals Armadillo Domain Proteins Base Sequence Binding Sites/genetics Brain/metabolism Cadherins/genetics,isolation & purification,physiology Catenins Cell Adhesion/physiology Cell Adhesion Molecules/chemistry,genetics,physiology Cell Line Cell Movement/physiology Cell Size/physiology Cytoskeletal Proteins/chemistry,genetics,isolation & purification,physiology DNA Primers/genetics Dogs Drosophila Proteins Humans Insect Proteins/chemistry,genetics Mice Molecular Sequence Data Nerve Tissue Proteins/chemistry,genetics,physiology Phosphoproteins Trans-Activators Transfection beta Catenin
Chemicals
Armadillo Domain Proteins CTNNB1 protein, human CTNNB1 protein, mouse Cadherins Catenins Cell Adhesion Molecules Cytoskeletal Proteins DNA Primers Drosophila Proteins Insect Proteins Nerve Tissue Proteins Phosphoproteins Trans-Activators beta Catenin delta catenin
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Lu Q
Center for Neurologic Diseases, Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Paredes M
Medina M
Zhou J
Cavallo R
Peifer M
Orecchio L
Kosik K S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.144.3.519
Published
1999-02-08
Pages
519-32
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2132907
Subset
IM
Grants
NIA NIH HHS · AG06601 · United States
NIGMS NIH HHS · GM47857 · United States
Databases
GENBANK
U96136
External Links
PubMed source DOI: 10.1083/jcb.144.3.519 PMC Full Text View journal details
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