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PMID: 7962096 Published · ppublish English Journal Article

Beta-catenin mediates the interaction of the cadherin-catenin complex with epidermal growth factor receptor.

The Journal of cell biology ·Vol. 127 ·No. 5 ·1994-12-00 ·Pages 1375-80

Hoschuetzky H, Aberle H, Kemler R

Abstract

Catenins mediate the linkage of classical cadherins with actin microfilaments and are part of a higher order protein structure by which cadherins are connected to other cytoplasmic and transmembrane proteins. The ratio of actin-bound to free cadherin-catenin complex, which varies depending on the type and growth rate of cells, is thought to be altered by cellular signals, such as those associated with mitosis, polarization of cells and growth factors during development. EGF induces an immediate tyrosine phosphorylation of beta-catenin and gamma-catenin (plakoglobin). We show here an association of the EGF-receptor with the cadherin-catenin complex. Using recombinant proteins we demonstrate the interaction of EGF-receptor and beta-catenin in in vitro kinase assays. This interaction is mediated by the evolutionarily conserved central "core" region of beta-catenin. These results suggest that catenins represent an important link between EGF-induced signal transduction and cadherin function.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cadherins/metabolism Cell Line Cell Polarity Cytoskeletal Proteins/metabolism Desmoplakins Epidermal Growth Factor/pharmacology ErbB Receptors/metabolism Humans Molecular Sequence Data Phosphorylation Recombinant Fusion Proteins/metabolism Recombinant Proteins/metabolism Signal Transduction Trans-Activators Tumor Cells, Cultured Tyrosine/metabolism beta Catenin gamma Catenin
Chemicals
CTNNB1 protein, human Cadherins Cytoskeletal Proteins Desmoplakins JUP protein, human Recombinant Fusion Proteins Recombinant Proteins Trans-Activators beta Catenin gamma Catenin Tyrosine Epidermal Growth Factor ErbB Receptors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hoschuetzky H
Max-Planck-Institut für Immunobiologie, Freiburg, Germany.
Aberle H
Kemler R
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.127.5.1375
Published
1994-12-00
Pages
1375-80
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120252
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.127.5.1375 PMC Full Text View journal details
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