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PMID: 9927434 Published · ppublish English Journal Article

Retention of empty MHC class I molecules by tapasin is essential to reconstitute antigen presentation in invertebrate cells.

The EMBO journal ·Vol. 18 ·No. 3 ·1999-02-01 ·Pages 743-53

Schoenhals GJ, Krishna RM, Grandea AG, Spies T, Peterson PA, Yang Y, Früh K

Abstract

Presentation of antigen-derived peptides by major histocompatibility complex (MHC) class I molecules is dependent on an endoplasmic reticulum (ER) resident glycoprotein, tapasin, which mediates their interaction with the transporter associated with antigen processing (TAP). Independently of TAP, tapasin was required for the presentation of peptides targeted to the ER by signal sequences in MHC class I-transfected insect cells. Tapasin increased MHC class I peptide loading by retaining empty but not peptide-containing MHC class I molecules in the ER. Upon co-expression of TAP, this retention/release function of tapasin was sufficient to reconstitute MHC class I antigen presentation in insect cells, thus defining the minimal non-housekeeping functions required for MHC class I antigen presentation.

MeSH Terms
ATP Binding Cassette Transporter, Subfamily B, Member 2 ATP-Binding Cassette Transporters/chemistry,metabolism Amino Acid Sequence Animals Antigen Presentation Antigens/genetics,metabolism Antiporters/metabolism Cell Line Dimerization Drosophila melanogaster Endoplasmic Reticulum/immunology,metabolism Genes, MHC Class I Histocompatibility Antigens Class I/metabolism Immunoglobulins/metabolism Membrane Transport Proteins Molecular Chaperones/metabolism Molecular Sequence Data Ovalbumin/genetics,immunology Protein Conformation Transfection
Chemicals
ATP Binding Cassette Transporter, Subfamily B, Member 2 ATP-Binding Cassette Transporters Antigens Antiporters Histocompatibility Antigens Class I Immunoglobulins Membrane Transport Proteins Molecular Chaperones TAP1 protein, human tapasin Ovalbumin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Schoenhals G J
The R.W.Johnson Pharmaceutical Research Institute, 3535 General Atomics Court Suite 100, San Diego, CA, 92121, USA.
Krishna R M
Grandea A G
Spies T
Peterson P A
Yang Y
Früh K
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/18.3.743
Published
1999-02-01
Pages
743-53
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1171167
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/18.3.743 PMC Full Text View journal details
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