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PMID: 992668 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Preparation of delipidized serum protein for use in cell culture systems.

In vitro ·Vol. 12 ·No. 8 ·1976-08-00 ·Pages 554-7

Rothblat GH, Arbogast LY, Ouellette L, Howard BV

Abstract

A rapid procedure for the preparation of delipidized serum protein is described. The delipidized protein can be used for the maintenance and growth of tissue culture cells in a lipid-free environment. The extraction procedure greatly reduces all serum lipid classes and the delipidized protein supports the growth of a variety of cells in culture.

MeSH Terms

Blood Proteins/isolation & purification Cell Division Cell Line Culture Media Methods

Chemicals

Blood Proteins Culture Media

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Rothblat G H

Arbogast L Y

Ouellette L

Howard B V

References (17)

17 references, click to expand

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Article Info

Journal

In vitro

Abbr.

In Vitro

ISSN

0073-5655

DOI

10.1007/BF02797438

Published

1976-08-00

Pages

554-7

Language

English

Region

United States

NLM ID

0063733

Subset

External Links

PubMed source DOI: 10.1007/BF02797438

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Preparation of delipidized serum protein for use in cell culture systems.

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