Desmosterol as the major sterol in L-cell mouse fibroblasts grown in sterol-free culture medium.

Science (New York, N.Y.) ·Vol. 169 ·No. 3948 ·1970-08-28 ·Pages 880-2

Rothblat GH, Burns CH, Conner RL, Landrey JR

Abstract

The principal sterol synthesized by L-cell mouse fibroblasts is desmosterol. Cholesterol was not detected in these cells when they were grown in a sterol-free culture medium. These findings indicate that, in cells, cholesterol can be replaced by desmosterol. Sterol analyses of six other tissue culture cell lines revealed cholesterol synthesis.

MeSH Terms

Acetates/metabolism Animals Carbon Isotopes Carcinoma Cholestanes/analysis,biosynthesis Cholesterol/biosynthesis Chromatography, Gas Chromatography, Thin Layer Culture Media In Vitro Techniques L Cells/metabolism Laryngeal Neoplasms Lung Mice Sterols/analysis,biosynthesis

Chemicals

Acetates Carbon Isotopes Cholestanes Culture Media Sterols Cholesterol

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Rothblat G H

Burns C H

Conner R L

Landrey J R

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.169.3948.880

Published

1970-08-28

Pages

880-2

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.169.3948.880 View journal details

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Desmosterol as the major sterol in L-cell mouse fibroblasts grown in sterol-free culture medium.

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