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PMID: 9882649 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Assembly of XcpR in the cytoplasmic membrane is required for extracellular protein secretion in Pseudomonas aeruginosa.

Journal of bacteriology ·Vol. 181 ·No. 2 ·1999-01-00 ·Pages 382-8

Ball G, Chapon-Hervé V, Bleves S, Michel G, Bally M

Abstract

A broad range of extracellular proteins secreted by Pseudomonas aeruginosa use the type II or general secretory pathway (GSP) to reach the medium. This pathway requires the expression of at least 12 xcp gene products. XcpR, a putative nucleotide-binding protein, is essential for the secretion process across the outer membrane even though the protein contains no hydrophobic sequence that could target or anchor it to the bacterial envelope. For a better understanding of the relationship between XcpR and the other Xcp proteins which are located in the envelope, we have studied its subcellular localization. In a wild-type P. aeruginosa strain, XcpR was found associated with the cytoplasmic membrane. This association depends on the presence of the XcpY protein, which also appears to be necessary for XcpR stability. Functional complementation of an xcpY mutant required the XcpY protein to be expressed at a low level. Higher expression precluded the complementing activity of XcpY, although membrane association of XcpR was restored. This behavior suggested that an excess of free XcpY might interfere with the secretion by formation of inactive XcpR-XcpY complexes which cannot properly interact with their natural partners in the secretion machinery. These data show that a precise stoichiometric ratio between several components may be crucial for the functioning of the GSP.

MeSH Terms
Bacterial Proteins/genetics,metabolism Cell Fractionation Cell Membrane/metabolism Genetic Complementation Test Membrane Transport Proteins Plasmids Pseudomonas aeruginosa/genetics,metabolism Recombinant Fusion Proteins/metabolism Restriction Mapping
Chemicals
Bacterial Proteins Membrane Transport Proteins Recombinant Fusion Proteins xcpR protein, Pseudomonas aeruginosa xcpY protein, Pseudomonas aeruginosa
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ball G
Laboratoire d'Ingénierie des Systèmes Macromoléculaires, Centre National de la Recherche Scientifique, 13402 Marseille Cedex 20, France.
Chapon-Hervé V
Bleves S
Michel G
Bally M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.2.382-388.1999
Published
1999-01-00
Pages
382-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC93389
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.181.2.382-388.1999 PMC Full Text View journal details
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