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PMID: 9850339 Published · ppublish English Journal Article

Human herpes virus 8 (HHV-8) in Kaposi's sarcoma: lack of association with Bcl-2 and p53 protein expression.

Molecular pathology : MP ·Vol. 51 ·No. 3 ·1998-06-00 ·Pages 155-9

Kennedy MM, O'Leary JJ, Oates JL, Lucas SB, Howells DD, Picton S, McGee JO

Abstract

Human herpes virus 8 (HHV-8) is the infectious agent implicated in the pathogenesis of Kaposi's sarcoma, although its mode of action is unclear. Recent work indicates that the HHV-8 genome encodes a viral Bcl-2 homologue (v-Bcl-2). The aim of this study was to explore Bcl-2 expression in Kaposi's sarcoma using a unique set of HHV-8 positive and negative cases, and to determine whether there is a relation with p53 expression. Up to 18 specimens from 17 patients were selected. HHV-8 status was determined using nested polymerase chain reaction (PCR) to the open reading frame (ORF) 26, with further confirmation by TaqMan PCR. In addition, Bcl-2 and p53 immunohistochemistry were performed using standard protocols. The results suggest that Bcl-2 and p53 expression is independent of HHV-8 status. In addition, there does not appear to be a direct correlation with disease stage. HHV8 histopathogenesis is likely to be a multifactorial complex process, which may be mediated in part by viral genes and apoptosis regulating homologues.

MeSH Terms
Female Herpesvirus 8, Human/isolation & purification Humans Immunoenzyme Techniques Male Neoplasm Proteins/metabolism Polymerase Chain Reaction Proto-Oncogene Proteins c-bcl-2/metabolism Sarcoma, Kaposi/metabolism,virology Tumor Suppressor Protein p53/metabolism
Chemicals
Neoplasm Proteins Proto-Oncogene Proteins c-bcl-2 Tumor Suppressor Protein p53
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kennedy M M
Nuffield Department of Pathology and Bacteriology, University of Oxford, UK.
O'Leary J J
Oates J L
Lucas S B
Howells D D
Picton S
McGee J O
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Article Info
Journal
Molecular pathology : MP
Abbr.
Mol Pathol
ISSN
1366-8714
DOI
10.1136/mp.51.3.155
Published
1998-06-00
Pages
155-9
Language
English
Region
England
NLM ID
9706282
PMCID
PMC395628
Subset
IM
External Links
PubMed source DOI: 10.1136/mp.51.3.155 PMC Full Text
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