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PMID: 9231158 Published · ppublish English Journal Article

HHV8 and Kaposi's sarcoma: a time cohort study.

Molecular pathology : MP ·Vol. 50 ·No. 2 ·1997-04-00 ·Pages 96-100

Kennedy MM, Lucas SB, Jones RR, Howells DD, Picton SJ, Hanks EE, McGee JO, O'Leary JJ

Abstract

The recent finding that human herpes virus 8 (HHV8) is found in the majority of Kaposi's sarcoma (KS) cases supports the epidemiological observation that the tumour may be caused by an infectious agent. This study aimed to address when and how HHV8 evolved. A cohort of African endemic KS (49 samples from 45 patients) and European KS (18 samples from 13 patients), spanning 27 years, was assessed for the presence of HHV8 by both standard solution phase polymerase chain reaction (PCR) and the newly described technique of TaqMan PCR. HHV8 was present in approximately 49% (24 of 49 tissue samples) of the African cases and in more than 90% (16 of 18 tissue samples) of the European cohort, in keeping with recent seroepidemiological data. HHV8 is strongly linked to the development of KS; however, in some patients, other factors may operate. The utility and flexibility of TaqMan PCR in detecting low copy viral target in human tissues was demonstrated.

MeSH Terms
Adolescent Adult Aged Aged, 80 and over Child Child, Preschool Cohort Studies Europe Female HIV Infections/complications Herpesvirus 8, Human/isolation & purification Humans Infant Infant, Newborn Malawi Male Middle Aged Polymerase Chain Reaction/methods Sarcoma, Kaposi/complications,virology
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Kennedy M M
Nuffield Department of Pathology & Bacteriology, University of Oxford, United Kingdom.
Lucas S B
Jones R R
Howells D D
Picton S J
Hanks E E
McGee J O
O'Leary J J
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Article Info
Journal
Molecular pathology : MP
Abbr.
Mol Pathol
ISSN
1366-8714
DOI
10.1136/mp.50.2.96
Published
1997-04-00
Pages
96-100
Language
English
Region
England
NLM ID
9706282
PMCID
PMC379591
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1136/mp.50.2.96 PMC Full Text
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