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PMID: 9835552 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation of marine polycyclic aromatic hydrocarbon (PAH)-degrading Cycloclasticus strains from the Gulf of Mexico and comparison of their PAH degradation ability with that of puget sound Cycloclasticus strains.

Applied and environmental microbiology ·Vol. 64 ·No. 12 ·1998-12-00 ·Pages 4703-10

Geiselbrecht AD, Hedlund BP, Tichi MA, Staley JT

Abstract

Phenanthrene- and naphthalene-degrading bacteria were isolated from four offshore and nearshore locations in the Gulf of Mexico by using a modified most-probable-number technique. The concentrations of these bacteria ranged from 10(2) to 10(6) cells per ml of wet surficial sediment in mildly contaminated and noncontaminated sediments. A total of 23 strains of polycyclic aromatic hydrocarbon (PAH)-degrading bacteria were obtained. Based on partial 16S ribosomal DNA sequences and phenotypic characteristics, these 23 strains are members of the genus Cycloclasticus. Three representatives were chosen for a complete phylogenetic analysis, which confirmed the close relationship of these isolates to type strain Cycloclasticus pugetii PS-1, which was isolated from Puget Sound. PAH substrate utilization tests which included high-molecular-weight PAHs revealed that these isolates had similar, broad substrate ranges which included naphthalene, substituted naphthalenes, phenanthrene, biphenyl, anthracene, acenaphthene, and fluorene. Degradation of pyrene and fluoranthene occurred only when the strains were incubated with phenanthrene. Two distinct partial PAH dioxygenase iron sulfur protein (ISP) gene sequences were PCR amplified from Puget Sound and Gulf of Mexico Cycloclasticus strains. Phylogenetic analyses of these sequences revealed that one ISP type is related to the bph type of ISP sequences, while the other ISP type is related to the nah type of ISP sequences. The predicted ISP amino acid sequences for the Gulf of Mexico and Puget Sound strains are identical, which supports the hypothesis that these geographically separated isolates are closely related phylogentically. Cycloclasticus species appear to be numerically important and widespread PAH-degrading bacteria in both Puget Sound and the Gulf of Mexico.

MeSH Terms
Bacteria/classification,isolation & purification,metabolism Biodegradation, Environmental Caribbean Region DNA, Ribosomal/genetics Naphthalenes/metabolism Oceans and Seas Oxygenases/genetics,metabolism Pacific Ocean Phenanthrenes/metabolism Phylogeny Polymerase Chain Reaction/methods RNA, Ribosomal, 16S/genetics,isolation & purification Species Specificity
Chemicals
DNA, Ribosomal Naphthalenes Phenanthrenes RNA, Ribosomal, 16S Oxygenases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Geiselbrecht A D
Department of Microbiology, University of Washington, Seattle, Washington 98195, USA. Allison.Geiselbrecht@noaa.gov
Hedlund B P
Tichi M A
Staley J T
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.64.12.4703-4710.1998
Email
allison.geiselbrecht@noaa.gov
Published
1998-12-00
Pages
4703-10
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC90912
Subset
IM
Grants
PHS HHS · 6M-0837-05 · United States
Databases
GENBANK
AF092998, AF092999, AF093000, AF093001, AF093002, AF093003, AF093004
External Links
PubMed source DOI: 10.1128/AEM.64.12.4703-4710.1998 PMC Full Text View journal details
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