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PMID: 9817886 Published · ppublish English Journal Article

Molecular epidemiology of ampicillin-resistant non-beta-lactamase-producing Haemophilus influenzae.

Journal of clinical microbiology ·Vol. 36 ·No. 12 ·1998-12-00 ·Pages 3629-35

Gazagne L, Delmas C, Bingen E, Dabernat H

Abstract

Resistance to ampicillin without beta-lactamase production is not a frequent occurrence among Haemophilus influenzae strains. This kind of resistance is encountered in unencapsulated strains isolated from bronchial secretions and ear, nose, and throat specimens and is exceptional in H. influenzae type b. We studied 29 of these strains from various areas in France and 2 reference strains. Strains were compared by using ribotyping, arbitarily primed PCR with two primers, and pulsed-field gel electrophoresis. Each technique enabled the identification of 20 to 23 different patterns among the 31 strains. The combination of the different patterns for the strains obtained by the different techniques provided 27 distinct profiles. According to these results, it seems that the clonal propagation of these resistant strains does not occur.

MeSH Terms
Adolescent Adult Aged Ampicillin Resistance Child Child, Preschool Electrophoresis, Gel, Pulsed-Field Haemophilus influenzae/drug effects,genetics Humans Infant Microbial Sensitivity Tests Middle Aged Polymerase Chain Reaction
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gazagne L
Laboratoire de Microbiologie, Centre Hospitalier Universitaire Purpan, Toulouse, France.
Delmas C
Bingen E
Dabernat H
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.36.12.3629-3635.1998
Published
1998-12-00
Pages
3629-35
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC105253
Subset
IM
External Links
PubMed source DOI: 10.1128/JCM.36.12.3629-3635.1998 PMC Full Text View journal details
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