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PMID: 9813090 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Time-lapse microscopy reveals unique roles for kinesins during anaphase in budding yeast.

The Journal of cell biology ·Vol. 143 ·No. 3 ·1998-11-02 ·Pages 687-94

Straight AF, Sedat JW, Murray AW

Abstract

The mitotic spindle is a complex and dynamic structure. Genetic analysis in budding yeast has identified two sets of kinesin-like motors, Cin8p and Kip1p, and Kar3p and Kip3p, that have overlapping functions in mitosis. We have studied the role of three of these motors by video microscopy of motor mutants whose microtubules and centromeres were marked with green fluorescent protein. Despite their functional overlap, each motor mutant has a specific defect in mitosis: cin8Delta mutants lack the rapid phase of anaphase B, kip1Delta mutants show defects in the slow phase of anaphase B, and kip3Delta mutants prolong the duration of anaphase to the point at which the spindle becomes longer than the cell. The kip3Delta and kip1Delta mutants affect the duration of anaphase, but cin8Delta does not.

MeSH Terms
Anaphase/physiology Fungal Proteins/metabolism Kinesins/metabolism,physiology Microtubule-Associated Proteins/metabolism Mitosis Molecular Motor Proteins Mutagenesis Photomicrography Saccharomyces cerevisiae Proteins Saccharomycetales/genetics,physiology,ultrastructure Spindle Apparatus Time Factors
Chemicals
CIN8 protein, S cerevisiae Fungal Proteins KIP1 protein, S cerevisiae KIP3 protein, S cerevisiae Microtubule-Associated Proteins Molecular Motor Proteins Saccharomyces cerevisiae Proteins Kinesins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Straight A F
Department of Physiology, School of Medicine, University of California San Francisco, San Francisco, California 94143, USA. aaron_straight@hms.harvard.edu
Sedat J W
Murray A W
References (50)
50 references, click to expand
  1. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  2. Loss of function of Saccharomyces cerevisiae kinesin-related CIN8 and KIP1 is suppressed by KAR3 motor domain mutations.
    Genetics. 1993 Sep;135(1):35-44 PMID: 8224825
  3. Chromosome instability mutants of Saccharomyces cerevisiae that are defective in microtubule-mediated processes.
    Mol Cell Biol. 1990 Jan;10(1):223-34 PMID: 2403635
  4. KAR3, a kinesin-related gene required for yeast nuclear fusion.
    Cell. 1990 Mar 23;60(6):1029-41 PMID: 2138512
  5. Mitotic motors.
    J Cell Biol. 1991 Nov;115(3):577-85 PMID: 1918154
  6. Direct experimental evidence for the existence, structural basis and function of astral forces during anaphase B in vivo.
    J Cell Sci. 1991 Oct;100 ( Pt 2):279-88 PMID: 1757488
  7. Kinesin-related cut7 protein associates with mitotic and meiotic spindles in fission yeast.
    Nature. 1992 Mar 5;356(6364):74-6 PMID: 1538784
  8. Suppression of a myosin defect by a kinesin-related gene.
    Nature. 1992 Mar 26;356(6367):358-61 PMID: 1549181
  9. Multiple kinesin-related proteins in yeast mitosis.
    Cold Spring Harb Symp Quant Biol. 1991;56:693-703 PMID: 1819517
  10. Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly.
    J Cell Biol. 1992 Jul;118(1):109-20 PMID: 1618897
  11. Kinesin-related proteins required for assembly of the mitotic spindle.
    J Cell Biol. 1992 Jul;118(1):95-108 PMID: 1618910
  12. Kinesin-related proteins required for structural integrity of the mitotic spindle.
    Cell. 1992 Aug 7;70(3):451-8 PMID: 1643659
  13. Astral and spindle forces in PtK2 cells during anaphase B: a laser microbeam study.
    J Cell Sci. 1993 Apr;104 ( Pt 4):1207-16 PMID: 8314902
  14. Cytoplasmic dynein plays a role in mammalian mitotic spindle formation.
    J Cell Biol. 1993 Nov;123(4):849-58 PMID: 8227145
  15. Disruption of mitotic spindle orientation in a yeast dynein mutant.
    Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10096-100 PMID: 8234262
  16. Cytoplasmic dynein is required for normal nuclear segregation in yeast.
    Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11172-6 PMID: 8248224
  17. Microtubule-dependent control of cell shape and pseudopodial activity is inhibited by the antibody to kinesin motor domain.
    J Cell Biol. 1993 Dec;123(6 Pt 2):1811-20 PMID: 8276899
  18. Immunofluorescence localization of the unconventional myosin, Myo2p, and the putative kinesin-related protein, Smy1p, to the same regions of polarized growth in Saccharomyces cerevisiae.
    J Cell Biol. 1994 May;125(4):825-42 PMID: 8188749
  19. Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends.
    EMBO J. 1994 Jun 1;13(11):2708-13 PMID: 7912193
  20. Rapid evolution of a protein in vitro by DNA shuffling.
    Nature. 1994 Aug 4;370(6488):389-91 PMID: 8047147
  21. Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro.
    J Cell Biol. 1995 Jan;128(1-2):107-15 PMID: 7822408
  22. Saccharomyces cerevisiae kinesin- and dynein-related proteins required for anaphase chromosome segregation.
    J Cell Biol. 1995 Feb;128(4):617-24 PMID: 7860634
  23. Spindle dynamics and cell cycle regulation of dynein in the budding yeast, Saccharomyces cerevisiae.
    J Cell Biol. 1995 Aug;130(3):687-700 PMID: 7622568
  24. Kinetics of spindle pole body separation in budding yeast.
    Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9707-11 PMID: 7568202
  25. Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces cerevisiae.
    J Cell Biol. 1995 Sep;130(6):1373-85 PMID: 7559759
  26. XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly.
    Cell. 1996 Jan 12;84(1):37-47 PMID: 8548824
  27. Force generation by microtubule assembly/disassembly in mitosis and related movements.
    Mol Biol Cell. 1995 Dec;6(12):1619-40 PMID: 8590794
  28. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer.
    Curr Biol. 1996 Feb 1;6(2):178-82 PMID: 8673464
  29. IVE (Image Visualization Environment): a software platform for all three-dimensional microscopy applications.
    J Struct Biol. 1996 Jan-Feb;116(1):56-60 PMID: 8742723
  30. Motors involved in spindle assembly and chromosome segregation.
    Curr Opin Cell Biol. 1996 Feb;8(1):4-9 PMID: 8791405
  31. Life with 6000 genes.
    Science. 1996 Oct 25;274(5287):546, 563-7 PMID: 8849441
  32. Forces acting on the fission yeast anaphase spindle.
    Cell Motil Cytoskeleton. 1996;34(1):69-75 PMID: 8860233
  33. The spindle assembly checkpoint.
    Curr Opin Cell Biol. 1996 Dec;8(6):773-80 PMID: 8939672
  34. GFP tagging of budding yeast chromosomes reveals that protein-protein interactions can mediate sister chromatid cohesion.
    Curr Biol. 1996 Dec 1;6(12):1599-608 PMID: 8994824
  35. Mutations that suppress the thermosensitivity of green fluorescent protein.
    Curr Biol. 1996 Dec 1;6(12):1653-63 PMID: 8994830
  36. How cells get the right chromosomes.
    Science. 1997 Jan 31;275(5300):632-7 PMID: 9005842
  37. APC-mediated proteolysis of Ase1 and the morphogenesis of the mitotic spindle.
    Science. 1997 Feb 28;275(5304):1311-4 PMID: 9036857
  38. 'Marker swap' plasmids: convenient tools for budding yeast molecular genetics.
    Yeast. 1997 Jun 15;13(7):647-53 PMID: 9200814
  39. Genetic studies of the lac repressor. V. Repressors which bind operator more tightly generated by suppression and reversion of nonsense mutations.
    J Mol Biol. 1978 Aug 15;123(3):431-54 PMID: 211238
  40. Mitotic spindle function in Saccharomyces cerevisiae requires a balance between different types of kinesin-related motors.
    Mol Biol Cell. 1997 Jun;8(6):1025-33 PMID: 9201713
  41. Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways.
    Mol Biol Cell. 1997 Jun;8(6):1035-50 PMID: 9201714
  42. Mitosis in living budding yeast: anaphase A but no metaphase plate.
    Science. 1997 Jul 25;277(5325):574-8 PMID: 9228009
  43. Spindle assembly in Xenopus egg extracts: respective roles of centrosomes and microtubule self-organization.
    J Cell Biol. 1997 Aug 11;138(3):615-28 PMID: 9245790
  44. Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex.
    J Cell Biol. 1997 Aug 11;138(3):629-41 PMID: 9245791
  45. The bimC family of kinesins: essential bipolar mitotic motors driving centrosome separation.
    Biochim Biophys Acta. 1997 Jul 24;1357(3):257-71 PMID: 9268050
  46. Kinesin-related KIP3 of Saccharomyces cerevisiae is required for a distinct step in nuclear migration.
    J Cell Biol. 1997 Sep 8;138(5):1023-40 PMID: 9281581
  47. Mitotic spindle positioning in Saccharomyces cerevisiae is accomplished by antagonistically acting microtubule motor proteins.
    J Cell Biol. 1997 Sep 8;138(5):1041-53 PMID: 9281582
  48. Astral microtubule dynamics in yeast: a microtubule-based searching mechanism for spindle orientation and nuclear migration into the bud.
    J Cell Biol. 1997 Nov 17;139(4):985-94 PMID: 9362516
  49. The Kar3p and Kip2p motors function antagonistically at the spindle poles to influence cytoplasmic microtubule numbers.
    J Cell Sci. 1998 Feb;111 ( Pt 3):295-301 PMID: 9427678
  50. The role of tubulin polymerization during spindle elongation in vitro.
    Cell. 1987 Apr 24;49(2):193-202 PMID: 2882855
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.143.3.687
Email
aaron_straight@hms.harvard.edu
Published
1998-11-02
Pages
687-94
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2148141
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.143.3.687 PMC Full Text View journal details
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