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PMID: 9811789 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Nuclear localization signal receptor importin alpha associates with the cytoskeleton.

The Plant cell ·Vol. 10 ·No. 11 ·1998-11-00 ·Pages 1791-9

Smith HM, Raikhel NV

Abstract

Importin alpha is the nuclear localization signal (NLS) receptor that is involved in the nuclear import of proteins containing basic NLSs. Using importin alpha as a tool, we were interested in determining whether the cytoskeleton could function in the transport of NLS-containing proteins from the cytoplasm to the nucleus. Double-labeling immunofluorescence studies showed that most of the cytoplasmic importin alpha coaligned with microtubules and microfilaments in tobacco protoplasts. Treatment of tobacco protoplasts with microtubule- or microfilament-depolymerizing agents disrupted the strands of importin alpha in the cytoplasm, whereas a microtubule-stabilizing agent had no effect. Biochemical analysis showed that importin alpha associated with microtubules and microfilaments in vitro in an NLS-dependent manner. The interaction of importin alpha with the cytoskeleton could be an essential element of protein transport from the cytoplasm to the nucleus in vivo.

MeSH Terms
Actin Cytoskeleton/metabolism Amino Acid Sequence Biological Transport, Active Cell Nucleus/metabolism Cytoskeleton/metabolism Karyopherins Microtubules/metabolism Molecular Sequence Data Nuclear Localization Signals/genetics,physiology Nuclear Proteins/metabolism Plant Proteins/metabolism Plants, Toxic Protoplasts/metabolism Tobacco/genetics,metabolism alpha Karyopherins
Chemicals
Karyopherins Nuclear Localization Signals Nuclear Proteins Plant Proteins alpha Karyopherins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Smith H M
Department of Energy-Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824-1312, USA.
Raikhel N V
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.10.11.1791
Published
1998-11-00
Pages
1791-9
Language
English
Region
England
NLM ID
9208688
PMCID
PMC143961
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.10.11.1791 PMC Full Text View journal details
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