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PMID: 9060466 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Microtubule-mediated transport of incoming herpes simplex virus 1 capsids to the nucleus.

The Journal of cell biology ·Vol. 136 ·No. 5 ·1997-03-10 ·Pages 1007-21

Sodeik B, Ebersold MW, Helenius A

Abstract

Herpes simplex virus 1 fuses with the plasma membrane of a host cell, and the incoming capsids are efficiently and rapidly transported across the cytosol to the nuclear pore complexes, where the viral DNA genomes are released into the nucleoplasm. Using biochemical assays, immunofluorescence, and immunoelectron microscopy in the presence and absence of microtubule depolymerizing agents, it was shown that the cytosolic capsid transport in Vero cells was mediated by microtubules. Antibody labeling revealed the attachment of dynein, a minus end-directed, microtubule-dependent motor, to the viral capsids. We propose that the incoming capsids bind to microtubules and use dynein to propel them from the cell periphery to the nucleus.

MeSH Terms
Actin Cytoskeleton/drug effects Animals Biological Transport Capsid/biosynthesis,metabolism Capsid Proteins Cell Nucleus/virology Chlorocebus aethiops Cytochalasin D/pharmacology Cytoplasm/virology Dyneins/analysis Herpesvirus 1, Human/metabolism Immediate-Early Proteins/biosynthesis Microtubules/drug effects,physiology Nocodazole/pharmacology Paclitaxel/pharmacology Vero Cells
Chemicals
Capsid Proteins Immediate-Early Proteins VP5 protein, Herpes simplex virus type 1 herpes simplex virus, type 1 protein ICP4 Cytochalasin D Dyneins Paclitaxel Nocodazole
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sodeik B
Yale University School of Medicine, Department of Cell Biology, New Haven, Connecticut 06520-8002, USA. sodeik@biomed.med.yale.edu
Ebersold M W
Helenius A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.136.5.1007
Email
sodeik@biomed.med.yale.edu
Published
1997-03-10
Pages
1007-21
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2132479
Subset
IM
Grants
NIAID NIH HHS · AI18599 · United States
External Links
PubMed source DOI: 10.1083/jcb.136.5.1007 PMC Full Text View journal details
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