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PMID: 9811652 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification and characterization of the fis operon in enteric bacteria.

Journal of bacteriology ·Vol. 180 ·No. 22 ·1998-11-00 ·Pages 5932-46

Beach MB, Osuna R

Abstract

The small DNA binding protein Fis is involved in several different biological processes in Escherichia coli. It has been shown to stimulate DNA inversion reactions mediated by the Hin family of recombinases, stimulate integration and excision of phage lambda genome, regulate the transcription of several different genes including those of stable RNA operons, and regulate the initiation of DNA replication at oriC. fis has also been isolated from Salmonella typhimurium, and the genomic sequence of Haemophilus influenzae reveals its presence in this bacteria. This work extends the characterization of fis to other organisms. Very similar fis operon structures were identified in the enteric bacteria Klebsiella pneumoniae, Serratia marcescens, Erwinia carotovora, and Proteus vulgaris but not in several nonenteric bacteria. We found that the deduced amino acid sequences for Fis are 100% identical in K. pneumoniae, S. marcescens, E. coli, and S. typhimurium and 96 to 98% identical when E. carotovora and P. vulgaris Fis are considered. The deduced amino acid sequence for H. influenzae Fis is about 80% identical and 90% similar to Fis in enteric bacteria. However, in spite of these similarities, the E. carotovora, P. vulgaris, and H. influenzae Fis proteins are not functionally identical. An open reading frame (ORF1) preceding fis in E. coli is also found in all these bacteria, and their deduced amino acid sequences are also very similar. The sequence preceding ORF1 in the enteric bacteria showed a very strong similarity to the E. coli fis P region from -53 to +27 and the region around -116 containing an ihf binding site. Both beta-galactosidase assays and primer extension assays showed that these regions function as promoters in vivo and are subject to growth phase-dependent regulation. However, their promoter strengths vary, as do their responses to Fis autoregulation and integration host factor stimulation.

MeSH Terms
Amino Acid Sequence Base Sequence Carrier Proteins/genetics DNA, Bacterial Enterobacteriaceae/genetics Escherichia coli Proteins Factor For Inversion Stimulation Protein Integration Host Factors Klebsiella pneumoniae/genetics Molecular Sequence Data Open Reading Frames Operon Pectobacterium carotovorum/genetics Promoter Regions, Genetic Proteus vulgaris/genetics Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Serratia marcescens/genetics Transcription, Genetic
Chemicals
Carrier Proteins DNA, Bacterial Escherichia coli Proteins Factor For Inversion Stimulation Protein Integration Host Factors integration host factor, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Beach M B
Department of Biological Sciences, University at Albany, Albany, New York 12222, USA.
Osuna R
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54 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.22.5932-5946.1998
Published
1998-11-00
Pages
5932-46
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC107668
Subset
IM
Grants
NIGMS NIH HHS · R01 GM052051 · United States
NIGMS NIH HHS · R29 GM052051 · United States
NIGMS NIH HHS · GM25051 · United States
Databases
GENBANK
AF040378, AF040379, AF040380, AF040381
External Links
PubMed source DOI: 10.1128/JB.180.22.5932-5946.1998 PMC Full Text View journal details
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