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PMID: 9733810 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Membrane fusion promoted by increasing surface densities of the paramyxovirus F and HN proteins: comparison of fusion reactions mediated by simian virus 5 F, human parainfluenza virus type 3 F, and influenza virus HA.

Journal of virology ·Vol. 72 ·No. 10 ·1998-10-00 ·Pages 7745-53

Dutch RE, Joshi SB, Lamb RA

Abstract

The membrane fusion reaction promoted by the paramyxovirus simian virus 5 (SV5) and human parainfluenza virus type 3 (HPIV-3) fusion (F) proteins and hemagglutinin-neuraminidase (HN) proteins was characterized when the surface densities of F and HN were varied. Using a quantitative content mixing assay, it was found that the extent of SV5 F-mediated fusion was dependent on the surface density of the SV5 F protein but independent of the density of SV5 HN protein, indicating that HN serves only a binding function in the reaction. However, the extent of HPIV-3 F protein promoted fusion reaction was found to be dependent on surface density of HPIV-3 HN protein, suggesting that the HPIV-3 HN protein is a direct participant in the fusion reaction. Analysis of the kinetics of lipid mixing demonstrated that both initial rates and final extents of fusion increased with rising SV5 F protein surface densities, suggesting that multiple fusion pores can be active during SV5 F protein-promoted membrane fusion. Initial rates and extent of lipid mixing were also found to increase with increasing influenza virus hemagglutinin protein surface density, suggesting parallels between the mechanism of fusion promoted by these two viral fusion proteins.

MeSH Terms
Animals Cell Line HN Protein/metabolism Hemagglutinin Glycoproteins, Influenza Virus/metabolism Humans Kinetics Membrane Fusion Microscopy, Confocal Respirovirus/metabolism Viral Fusion Proteins/metabolism
Chemicals
HN Protein Hemagglutinin Glycoproteins, Influenza Virus Viral Fusion Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dutch R E
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208-3500, USA.
Joshi S B
Lamb R A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.72.10.7745-7753.1998
Published
1998-10-00
Pages
7745-53
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC110082
Subset
IM
Grants
NIAID NIH HHS · F32 AI009607 · United States
NIAID NIH HHS · R01 AI023173 · United States
NIAID NIH HHS · AI-23173 · United States
NIAID NIH HHS · F32 AI-09607 · United States
External Links
PubMed source DOI: 10.1128/JVI.72.10.7745-7753.1998 PMC Full Text View journal details
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