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PMID: 9732283 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Degradation of misfolded endoplasmic reticulum glycoproteins in Saccharomyces cerevisiae is determined by a specific oligosaccharide structure.

The Journal of cell biology ·Vol. 142 ·No. 5 ·1998-09-07 ·Pages 1223-33

Jakob CA, Burda P, Roth J, Aebi M

Abstract

In Saccharomyces cerevisiae, transfer of N-linked oligosaccharides is immediately followed by trimming of ER-localized glycosidases. We analyzed the influence of specific oligosaccharide structures for degradation of misfolded carboxypeptidase Y (CPY). By studying the trimming reactions in vivo, we found that removal of the terminal alpha1,2 glucose and the first alpha1,3 glucose by glucosidase I and glucosidase II respectively, occurred rapidly, whereas mannose cleavage by mannosidase I was slow. Transport and maturation of correctly folded CPY was not dependent on oligosaccharide structure. However, degradation of misfolded CPY was dependent on specific trimming steps. Degradation of misfolded CPY with N-linked oligosaccharides containing glucose residues was less efficient compared with misfolded CPY bearing the correctly trimmed Man8GlcNAc2 oligosaccharide. Reduced rate of degradation was mainly observed for misfolded CPY bearing Man6GlcNAc2, Man7GlcNAc2 and Man9GlcNAc2 oligosaccharides, whereas Man8GlcNAc2 and, to a lesser extent, Man5GlcNAc2 oligosaccharides supported degradation. These results suggest a role for the Man8GlcNAc2 oligosaccharide in the degradation process. They may indicate the presence of a Man8GlcNAc2-binding lectin involved in targeting of misfolded glycoproteins to degradation in S. cerevisiae.

MeSH Terms
Base Sequence Carbohydrate Sequence Carboxypeptidases/metabolism Cathepsin A Endoplasmic Reticulum/enzymology Fungal Proteins/metabolism Glycoproteins/metabolism Kinetics Mannosidases/metabolism Molecular Sequence Data Mutagenesis/genetics Oligosaccharides/chemistry Protein Folding Protein Processing, Post-Translational/physiology Saccharomyces cerevisiae/enzymology alpha-Glucosidases/metabolism
Chemicals
Fungal Proteins Glycoproteins Oligosaccharides 4-nitrophenyl-alpha-glucosidase Mannosidases glucosidase I mannosyl-oligosaccharide 1,2-alpha-mannosidase alpha-Glucosidases Carboxypeptidases Cathepsin A
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jakob C A
Division of Cell and Molecular Pathology, Department of Pathology, University of Zürich, CH-8091 Zürich, Switzerland.
Burda P
Roth J
Aebi M
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
Published
1998-09-07
Pages
1223-33
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2149342
Subset
IM
Databases
GENBANK
Z49631, Z71645
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