Study of calcium dipicolinate release during bacterial spore germination by using a new, sensitive assay for dipicolinate.
The release of calcium and dipicolinic acid from spores of Bacillus megaterium KM during L-alanine-induced triggering of germination has been studied using a new, simple, and rapid assay for dipicolinic acid capable of detecting a concentration of 0.5 micron. The release of both calcium and dipicolinate started within seconds of exposure of the spores to L-alanine, thus preceding other measurable changes associated with germination. From the earliest times, the two substances were released in equimolar quantities, although later in germination calcium predominated.
11 references, click to expand
-
Rapid, chloramphenicol-resistant, activation of membrane electron transport on germination of Bacillus spores.Nature. 1977 Mar 10;266(5598):174-6 PMID: 404562
-
Rapid determination of dipicolinic acid in the spores of Clostridium species by gas-liquid chromatography.Appl Environ Microbiol. 1976 Jan;31(1):25-8 PMID: 942206
-
Heat resistance of bacterial endospores and concept of an expanded osmoregulatory cortex.Nature. 1975 Dec 4;258(5534):402-5 PMID: 1196370
-
Sequence of events during germination of putrefactive anaerobe 3679 spores.J Bacteriol. 1967 Sep;94(3):506-11 PMID: 4166552
-
Determination of dipicolinic acid in bacterial spores by ultraviolet spectrometry of the calcium chelate.Anal Biochem. 1967 May;19(2):327-37 PMID: 4963388
-
Water Permeability of Bacterial Spores and the Concept of a Contractile Cortex.Science. 1960 Aug 26;132(3426):544-5 PMID: 17798829
-
Colorimetric assay for dipicolinic acid in bacterial spores.Science. 1958 Jan 3;127(3288):26-7 PMID: 13495474
-
Kinetics of the release of dipicolinic acid from spores of Bacillus subtilis.J Bacteriol. 1958 Jul;76(1):81-3 PMID: 13563394
-
Alterations in metal content of spores of Bacillus megaterium and the effect on some spore properties.J Bacteriol. 1959 Jul;78(1):117-23 PMID: 13672918
-
Germination of bacterial endospores with calcium and dipicolinic acid.Science. 1961 May 26;133(3465):1703-4 PMID: 13741539
-
Sequence of events during rapid germination of spores of Bacillus cereus.J Gen Microbiol. 1971 Jan;65(1):101-4 PMID: 4996778
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
