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PMID: 4166552 Published · ppublish English Journal Article

Sequence of events during germination of putrefactive anaerobe 3679 spores.

Journal of bacteriology ·Vol. 94 ·No. 3 ·1967-09-00 ·Pages 506-11

Uehara M, Frank HA

Abstract

The sequence of changes during germination of putrefactive anaerobe 3679h spores was studied under aerobic conditions in a solution containing l-alanine and sodium pyrophosphate. Evidence that specific changes occurred in two distinct regions of the spore is given by data on several criteria that were used to measure germination. During the initial stage of germination, the absorbancy decreased, dipicolinic acid was released, the spores lost their resistance to heat and toxic chemicals, and the spore periphery (cortex) darkened gradually under phase-contrast microscopy. The final stage of germination was characterized by changes in the central spore region (core), notably phase darkening of the spore center and stainability with mercurochrome, and by a slight additional absorbancy decrease.

MeSH Terms
Alanine/pharmacology Clostridium/drug effects,growth & development Diphosphates/pharmacology Hot Temperature Microscopy, Phase-Contrast Oxygen Consumption Pimelic Acids/metabolism Spores Staining and Labeling
Chemicals
Diphosphates Pimelic Acids Alanine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Uehara M
Frank H A
References (17)
17 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1967-09-00
Pages
506-11
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC251914
Subset
IM
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