Abstract
The sequence of changes during germination of putrefactive anaerobe 3679h spores was studied under aerobic conditions in a solution containing l-alanine and sodium pyrophosphate. Evidence that specific changes occurred in two distinct regions of the spore is given by data on several criteria that were used to measure germination. During the initial stage of germination, the absorbancy decreased, dipicolinic acid was released, the spores lost their resistance to heat and toxic chemicals, and the spore periphery (cortex) darkened gradually under phase-contrast microscopy. The final stage of germination was characterized by changes in the central spore region (core), notably phase darkening of the spore center and stainability with mercurochrome, and by a slight additional absorbancy decrease.
MeSH Terms
Alanine/pharmacology
Clostridium/drug effects,growth & development
Diphosphates/pharmacology
Hot Temperature
Microscopy, Phase-Contrast
Oxygen Consumption
Pimelic Acids/metabolism
Spores
Staining and Labeling
Chemicals
Diphosphates
Pimelic Acids
Alanine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Uehara M
Frank H A
References (17)
17 references, click to expand
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