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PMID: 9707590 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The role of SOS and flap processing in microsatellite instability in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 17 ·1998-08-18 ·Pages 10003-8

Morel P, Reverdy C, Michel B, Ehrlich SD, Cassuto E

Abstract

Mutations affecting mismatch repair result in elevated frequencies of microsatellite length alteration in prokaryotes and eukaryotes. However, the finding that microsatellite instability is found often in cells with a functional mismatch repair system prompted a search for other factors of tract alteration. In the present report, we show that, in Escherichia coli, poly(AC/TG) tracts are destabilized by mutations that induce SOS. These observations may have implications for eukaryotic cells because recent results suggest the existence of a mammalian SOS response analogous to that in prokaryotes. In addition, a defect in the 5'-3' exonuclease domain of DNA polymerase I, homologous to the mammalian FEN1 and the yeast RAD27 nucleases, leads to a marked increase in repeat expansions characteristic of several genetic disorders. Finally, we found that the combination of a proofreading defect with mismatch repair deficiency results in extreme microsatellite instability.

MeSH Terms
Animals Base Sequence Checkpoint Kinase 1 DNA Primers/genetics DNA Repair DNA Replication DNA, Bacterial/genetics,metabolism Dinucleotide Repeats Endodeoxyribonucleases/metabolism Escherichia coli/genetics,metabolism Flap Endonucleases Frameshift Mutation Microsatellite Repeats Polymerase Chain Reaction Protein Kinases/genetics Recombination, Genetic SOS Response, Genetics
Chemicals
DNA Primers DNA, Bacterial Protein Kinases Checkpoint Kinase 1 Endodeoxyribonucleases Flap Endonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Morel P
Génétique Microbienne, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France.
Reverdy C
Michel B
Ehrlich S D
Cassuto E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.17.10003
Published
1998-08-18
Pages
10003-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC21451
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.95.17.10003 PMC Full Text View journal details
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