Home LiteratureArticle Details
PMID: 2671653 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genetic requirements for hyper-recombination by very short patch mismatch repair: involvement of Escherichia coli DNA polymerase I.

Molecular & general genetics : MGG ·Vol. 217 ·No. 2-3 ·1989-06-00 ·Pages 254-6

Dzidic S, Radman M

Abstract

It has been established that very short patch (VSP) mismatch repair, depending in Escherichia coli on MutL, MutS and Dcm functions, is responsible for the hyper-recombinogenic effect of a class of genetic markers. We show that VSP repair requires the presence of the complete DNA polymerase I enzyme. The absence of endonuclease activities involved in the repair of base-loss sites, Nth, Nfo and Xth, does not affect VSP repair. Implications for the mechanism of the VSP repair are discussed.

MeSH Terms
Base Composition Base Sequence Crosses, Genetic DNA Polymerase I/metabolism DNA Repair Escherichia coli/genetics,metabolism Genetic Markers Mutation Recombination, Genetic
Chemicals
Genetic Markers DNA Polymerase I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dzidic S
Institut Jacques Monod, Paris, France.
Radman M
References (19)
19 references, click to expand
  1. Endonuclease III (nth) mutants of Escherichia coli.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):474-8 PMID: 2982160
  2. Very short patch mismatch repair in phage lambda: repair sites and length of repair tracts.
    Genetics. 1986 Dec;114(4):1041-60 PMID: 2948873
  3. Heteroduplex deoxyribonucleic acid base mismatch repair in bacteria.
    Microbiol Rev. 1986 Jun;50(2):133-65 PMID: 3523187
  4. Are purine bases enzymatically inserted into depurinated DNA in Escherichia coli?
    J Biochem. 1982 Sep;92(3):971-3 PMID: 6754712
  5. DNA mismatch correction.
    Annu Rev Biochem. 1987;56:435-66 PMID: 3304141
  6. DNA repair enzymes.
    Annu Rev Biochem. 1982;51:61-87 PMID: 6287922
  7. Mismatch repair in Escherichia coli.
    Annu Rev Genet. 1986;20:523-38 PMID: 2949693
  8. Localized conversion in Streptococcus pneumoniae recombination: heteroduplex preference.
    Genetics. 1985 Aug;110(4):557-68 PMID: 4029607
  9. Mismatch repair and recombination in E. coli.
    Cell. 1987 Aug 14;50(4):621-6 PMID: 2955904
  10. Method for determining whether a gene of Escherichia coli is essential: application to the polA gene.
    J Bacteriol. 1984 May;158(2):636-43 PMID: 6233260
  11. Molecular basis of base substitution hotspots in Escherichia coli.
    Nature. 1978 Aug 24;274(5673):775-80 PMID: 355893
  12. DNA mismatch-repair in Escherichia coli counteracting the hydrolytic deamination of 5-methyl-cytosine residues.
    EMBO J. 1987 Jun;6(6):1809-15 PMID: 3038536
  13. Specific mismatch correction in bacteriophage lambda crosses by very short patch repair.
    Mol Gen Genet. 1983;191(1):118-25 PMID: 6225003
  14. Recombination in the lambda repressor gene: evidence that very short patch (VSP) mismatch correction restores a specific sequence.
    Mol Gen Genet. 1985;199(3):465-70 PMID: 2993796
  15. Bacterial genes mutL, mutS, and dcm participate in repair of mismatches at 5-methylcytosine sites.
    J Bacteriol. 1987 Nov;169(11):5241-6 PMID: 2959653
  16. Endonuclease IV (nfo) mutant of Escherichia coli.
    J Bacteriol. 1986 Dec;168(3):1120-7 PMID: 2430946
  17. Escherichia coli endonuclease III is not an endonuclease but a beta-elimination catalyst.
    Biochem J. 1987 Mar 1;242(2):565-72 PMID: 2439070
  18. Mismatch repair of deaminated 5-methyl-cytosine.
    J Mol Biol. 1987 Mar 5;194(1):155-9 PMID: 2956428
  19. Characterization of the action of Escherichia coli DNA polymerase I at incisions produced by repair endodeoxyribonucleases.
    J Biol Chem. 1982 Jan 10;257(1):575-83 PMID: 6273443
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF02464889
Published
1989-06-00
Pages
254-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02464889
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2