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PMID: 9707550 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Promoter-proximal stalling results from the inability to recruit transcription factor IIH to the transcription complex and is a regulated event.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 17 ·1998-08-18 ·Pages 9767-72

Kumar KP, Akoulitchev S, Reinberg D

Abstract

Promoter-proximal stalling, a general phenomenon observed during the expression of many RNA polymerase II transcribed genes, is dependent on transcription factor IIH (TFIIH). Reactions lacking TFIIH initiated transcription, but the transcription complex encountered a block to elongation proximal to the promoter. The accumulation of promoter-proximal stalled complexes was reduced in the presence of TFIIH and efficient escape from this site also required an activator. Promoter-proximal stalled complexes could not be induced to resume elongation. Our results indicate that effective recruitment of TFIIH into transcription complexes is achieved during formation of the preinitiation complex at the promoter. The studies establish that promoter clearance is a regulated event that requires TFIIH.

MeSH Terms
Adenosine Triphosphate/metabolism Base Sequence DNA/genetics,metabolism DNA-Binding Proteins/metabolism Promoter Regions, Genetic RNA Polymerase II/metabolism Transcription Factor TFIIH Transcription Factors/metabolism Transcription Factors, TFII Transcription, Genetic
Chemicals
DNA-Binding Proteins Transcription Factors Transcription Factors, TFII Transcription Factor TFIIH Adenosine Triphosphate DNA RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kumar K P
Howard Hughes Medical Institute, Division of Nucleic Acid Enzymology, University of Medicine and Dentistry of New Jersey, 663 Hoes Lane, Piscataway, NJ 08854-5635, USA.
Akoulitchev S
Reinberg D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.17.9767
Published
1998-08-18
Pages
9767-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC21411
Subset
IM
Grants
NIGMS NIH HHS · R01 GM037120 · United States
NIGMS NIH HHS · R37 GM037120 · United States
NIGMS NIH HHS · GM-37120 · United States
External Links
PubMed source DOI: 10.1073/pnas.95.17.9767 PMC Full Text View journal details
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