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PMID: 9696831 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Binding of Sindbis virus to cell surface heparan sulfate.

Journal of virology ·Vol. 72 ·No. 9 ·1998-09-00 ·Pages 7349-56

Byrnes AP, Griffin DE

Abstract

Alphaviruses are arthropod-borne viruses with wide species ranges and diverse tissue tropisms. The cell surface receptors which allow infection of so many different species and cell types are still incompletely characterized. We show here that the widely expressed glycosaminoglycan heparan sulfate can participate in the binding of Sindbis virus to cells. Enzymatic removal of heparan sulfate or the use of heparan sulfate-deficient cells led to a large reduction in virus binding. Sindbis virus bound to immobilized heparin, and this interaction was blocked by neutralizing antibodies against the viral E2 glycoprotein. Further experiments showed that a high degree of sulfation was critical for the ability of heparin to bind Sindbis virus. However, Sindbis virus was still able to infect and replicate on cells which were completely deficient in heparan sulfate, indicating that additional receptors must be involved. Cell surface binding of another alphavirus, Ross River virus, was found to be independent of heparan sulfate.

MeSH Terms
Animals CHO Cells Cell Line Cell Membrane/metabolism Chondroitin Sulfates/metabolism Cricetinae Heparitin Sulfate/metabolism Mice Mice, Inbred BALB C Polysaccharides/metabolism Receptors, Virus/metabolism Sindbis Virus/metabolism Sulfates Viral Plaque Assay
Chemicals
Polysaccharides Receptors, Virus Sulfates Chondroitin Sulfates Heparitin Sulfate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Byrnes A P
Departments of Molecular Microbiology and Immunology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205, USA.
Griffin D E
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.72.9.7349-7356.1998
Published
1998-09-00
Pages
7349-56
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC109959
Subset
IM
Grants
NINDS NIH HHS · R01 NS018596 · United States
NIAID NIH HHS · T32 AI007417 · United States
NINDS NIH HHS · R01-NS18596 · United States
NIAID NIH HHS · T32-AI07417 · United States
External Links
PubMed source DOI: 10.1128/JVI.72.9.7349-7356.1998 PMC Full Text View journal details
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