Home LiteratureArticle Details
PMID: 708264 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of a Sinbis virus variant with altered host range.

Archives of virology ·Vol. 58 ·No. 2 ·1978-00-00 ·Pages 127-36

Symington J, Schlesinger MJ

Abstract

A variant of Sindbis virus which is much more infectious for mouse cells than the standard virus has been examined for biochemical properties which might be responsible for this biological difference. The variant has a much enhanced ability to adsorb to mouse plasmacytoma (MOPC 315) cells, but when these cells were pretreated with heparin, they were able to adsorb the standard virus almost as well as the variant. This suggested that there was a surface charge difference between variant and standard virus. Differential elution of the viruses from hydroxyapatite and the results of isoelectric focusing of the virion glycoproteins substantiate this interpretation. Both viral glycoproteins E1 and E2 from the variant were more negatively charged than those of the standard virus but we were unable to find changes in tryptic peptides of the variant. Differences were found in stability of the two virus strains to heat and proteolytic enzymes.

MeSH Terms
Adsorption Cell Line Genetic Variation Glycoproteins/analysis Hemagglutination, Viral Heparin/pharmacology Isoelectric Focusing Sindbis Virus/analysis,physiology Surface Properties Viral Proteins/analysis
Chemicals
Glycoproteins Viral Proteins Heparin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Symington J
Schlesinger M J
References (24)
24 references, click to expand
  1. Isolation of a series of Mengo virus variants differing in sensitivity to polyanions.
    Can J Microbiol. 1967 Jun;13(6):651-7 PMID: 4291974
  2. Isolation and characterization of conditional-lethal mutants of Sindbis virus.
    Virology. 1966 Oct;30(2):204-13 PMID: 5919228
  3. Some investigations on the adjuvant mechanism of DEAE dextran.
    Arch Virol. 1975;47(3):225-35 PMID: 164161
  4. Virus-polysaccharide interactions. II. Enhancement of plague formation and the detection of variants of poliovirus with dextran sulfate.
    Virology. 1962 Jul;17 :499-501 PMID: 13919219
  5. Antigenic characterization of two sindbis envelope glycoproteins separated by isoelectric focusing.
    Virology. 1976 Jan;69(1):93-103 PMID: 54983
  6. Effects of polyions on Japanese encephalitis virus: difference in interaction of virus with DEAE dextran and dextran sulfate between PS cell adapted and non-adapted virus.
    Arch Gesamte Virusforsch. 1972;39(1):83-91 PMID: 4564456
  7. Glycoproteins of Sindbis virus: priliminary characterization of the oligosaccharides.
    J Virol. 1974 Sep;14(3):522-30 PMID: 4852175
  8. Formation of Sindbis virus proteins: identification of a precursor for one of the envelope proteins.
    J Virol. 1972 Nov;10(5):925-32 PMID: 4673887
  9. Mutant of polyoma virus with impaired adsorption to BHK cells.
    J Virol. 1974 Apr;13(4):931-4 PMID: 4362517
  10. Fractionation of biologically active and inactive populations of human rhinovirus type 2.
    Virology. 1975 Feb;63(2):384-94 PMID: 163526
  11. DINTER Z, PHILIPSON L: GENETIC MARKERS ASSOCIATED WITH VIRULENCE OF FOOT-AND-MOUTH DISEASE VIRUS.
    Proc Soc Exp Biol Med. 1963 Aug-Sep;113:1019-22 PMID: 14073097
  12. Purification of two plaque variants of sindbis virus.
    Arch Gesamte Virusforsch. 1970;31(3):200-6 PMID: 4992466
  13. Infectious virus-antibody complexes of sindbis virus.
    Infect Immun. 1977 Mar;15(3):720-5 PMID: 870428
  14. THE BASIS FOR THE INTERACTION BETWEEN ATTENUATED POLIOVIRUS AND POLYIONS.
    Virology. 1964 Dec;24:617-25 PMID: 14240411
  15. Virus particle adsorption, III. Adsorption of viruses by cell monolayers and effects of some variables on adsorption.
    Biochim Biophys Acta. 1960 Jun 3;40:400-10 PMID: 13792819
  16. Isolation of a Sindbis virus variant by passage on mouse plasmacytoma cells.
    J Virol. 1975 Apr;15(4):1037-41 PMID: 1167912
  17. Thermostability of Newcastle disease virus strains of different virulence.
    Arch Virol. 1975;47(3):249-55 PMID: 1168045
  18. HEPARIN AND RELATED POLYIONIC SUBSTANCES AS VIRUS INHIBITORS.
    Acta Pathol Microbiol Scand Suppl. 1964;:SUPPL 171:1-98 PMID: 14227873
  19. Kinetics of heat inactivation of Venezuelan equine encephalomyelitis virus.
    Arch Virol. 1976;51(4):307-17 PMID: 973799
  20. Separation of Sindbis virus plaque-type variants by calcium phosphate chromatography.
    Arch Gesamte Virusforsch. 1970;29(1):83-9 PMID: 5452662
  21. Peptide composition of sindbis virus variants.
    Arch Gesamte Virusforsch. 1970;31(3):207-14 PMID: 5490642
  22. Effect of heat on virions of wild-type Sindbis virus and a thermostable mutant.
    Virology. 1968 Feb;34(2):361-4 PMID: 5640824
  23. Studies of the early events of the replicative cycle of three variants of Mengo encephalomyelitis virus in mouse fibroblast cells.
    Virology. 1970 Dec;42(4):1087-96 PMID: 4322160
  24. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses.
    Am J Trop Med Hyg. 1958 Sep;7(5):561-73 PMID: 13571577
Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01315405
Published
1978-00-00
Pages
127-36
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01315405 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2